The Peculiar Structure of Acetobacter pasteurianus CIP103108 LPS Core Oligosaccharide. (29th October 2020)

• Record Type: Journal Article
• Authors: Marchetti, Roberta; Nieto Fabregat, Ferran; Pallach, Mateusz; Gully, Djamel; Giraud, Eric; Molinaro, Antonio; Duda, Katarzyna A.; Silipo, Alba
• Is Part Of: Chembiochem. Volume 22:Number 1(2021)
• Page Start: 147
• Page End: 150
• Publication Date: 2020-10-29
• Subjects: acetic acid bacteria -- Acetobacter pasteurianus -- core oligosaccharide -- lipopolysaccharide -- NMR spectroscopy; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202000597
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Acetobacter pasteurianus, a member of the Alphaproteobacteria, is an acetic acid‐producing bacterium present on sugar‐rich substrates such as such as fruits, flowers and vegetables and traditionally used in the production of fermented food. The preferred living habitat associated with acid conditions makes the structure of the bacterial cell wall interesting to study, due to expected uncommon features. We have used a combination of chemical, analytical and NMR spectroscopy approaches to define the complete structure of the core oligosaccharide from A. pasteurianus CIP103108 LPS. Interestingly, the core oligosaccharide displays a high concentration of negatively charged groups, structural features that might contribute to reinforcing the bacterial membrane. Abstract : Core values : A. pasteurianus is is an acetic acid‐producing bacterium. Herein, we report the structure of the A. pasteurianus CIP103108 LPS core oligosaccharide, rich of negatively charged residues, like phosphate groups, Kdo, Ko and glucuronic acid.