Nuclear factor kappa B inhibitor suppresses experimental autoimmune neuritis in mice via declining macrophages polarization to M1 type. (6th July 2021)

• Record Type: Journal Article
• Authors: Shen, Donghui; Chu, Fengna; Lang, Yue; Zheng, Chao; Li, Chunrong; Liu, Kangding; Zhu, Jie
• Is Part Of: Clinical and experimental immunology. Volume 206:Number 1(2021)
• Page Start: 110
• Page End: 117
• Publication Date: 2021-07-06
• Subjects: BAY11‐7082 -- EAN -- GBS -- macrophages -- NF‐κB; Immunopathology -- Periodicals; 616.079
• DOI: 10.1111/cei.13637
• Languages: English
• ISSNs: 0009-9104

Abstract

Abstract: Guillain–Barré syndrome (GBS) is an acute inflammatory and immune‐mediated demyelinating disease of the peripheral nervous system (PNS). Macrophages play a central role in its animal model, experimental autoimmune neuritis (EAN), which has been well accepted. Additionally, nuclear factor (NF)‐κB inhibitors have been used to treat cancers and have shown beneficial effects. Here, we investigated the therapeutic effect of M2 macrophage and the NF‐κB pathway's correlation with macrophage activation in EAN in C57BL/6 mice. We demonstrate that M2 macrophage transfusion could alleviate the clinical symptoms of EAN by reducing the proportion of M1 macrophage in the peak period, inhibiting the phosphorylation of NF‐κB p65. The NF‐κB inhibitor (BAY‐11‐7082) could alleviate the clinical symptoms of EAN and shorten the duration of symptoms by reducing the proportion of M1 macrophages and the expression of proinflammatory cytokines. Consequently, BAY‐11‐7082 exhibits strong potential as a therapeutic strategy for ameliorating EAN by influencing the balance of M1/M2 macrophages and inflammatory cytokines. Abstract : M2 macrophages were induced in vitro successfully. M2 macrophages were transferred to EAN and ameliorated the severity of EAN. Percentage of M1 macrophages was reduced and the M2 macrophages were increased in spleen MNCs of M2 macrophages‐treated group compared to the control group. M2 macrophages treatment had reduced the level of p‐p65 and decreased pro‐inflammatory cytokines greatly compared to the control group.