Ascorbate accumulation during sulphur deprivation and its effects on photosystem II activity and H2 production of the green alga Chlamydomonas reinhardtii. (13th April 2016)

• Record Type: Journal Article
• Authors: Nagy, Valéria; Vidal‐Meireles, André; Tengölics, Roland; Rákhely, Gábor; Garab, Győző; Kovács, László; Tóth, Szilvia Z.
• Is Part Of: Plant, cell and environment. Volume 39:Number 7(2016)
• Page Start: 1460
• Page End: 1472
• Publication Date: 2016-04-13
• Subjects: chlorophyll a fluorescence -- hydrogenase -- oxygen evolution -- thermoluminescence; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.12701
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: In nature, H2 production in Chlamydomonas reinhardtii serves as a safety valve during the induction of photosynthesis in anoxia, and it prevents the over‐reduction of the photosynthetic electron transport chain. Sulphur deprivation of C. reinhardtii also triggers a complex metabolic response resulting in the induction of various stress‐related genes, down‐regulation of photosynthesis, the establishment of anaerobiosis and expression of active hydrogenase. Photosystem II (PSII) plays dual role in H2 production because it supplies electrons but the evolved O2 inhibits the hydrogenase. Here, we show that upon sulphur deprivation, the ascorbate content in C. reinhardtii increases about 50‐fold, reaching the mM range; at this concentration, ascorbate inactivates the Mn‐cluster of PSII, and afterwards, it can donate electrons to tyrozin Z + at a slow rate. This stage is followed by donor‐side‐induced photoinhibition, leading to the loss of charge separation activity in PSII and reaction centre degradation. The time point at which maximum ascorbate concentration is reached in the cell is critical for the establishment of anaerobiosis and initiation of H2 production. We also show that ascorbate influenced H2 evolution via altering the photosynthetic electron transport rather than hydrogenase activity and starch degradation. Abstract : Sulphur deprivation of sealed C. reinhardtii cultures results in the down‐regulation of photosynthesis, establishment of anaerobiosis and expression of hydrogenases. Photosystem II has a determining role in H2 production because it supplies electrons but the evolved O2 inhibits the hydrogenase. Here, we show that upon sulphur deprivation, the ascorbate content in C. reinhardtii increases dramatically, and in the mM range, it inactivates the oxygen‐evolving complex. Therefore, we propose that photosystem II inactivation upon sulphur deprivation is initiated by a strong ascorbate accumulation, and it occurs via donor‐side‐induced photoinhibition.