A multicomponent reaction platform towards multimodal near-infrared BODIPY dyes for STED and fluorescence lifetime imaging. Issue 10 (1st September 2022)

• Record Type: Journal Article
• Authors: Fernandez, Antonio; Kielland, Nicola; Makda, Ashraff; Carragher, Neil O.; González-García, M. Carmen; Espinar-Barranco, Laura; González-Vera, Juan A.; Orte, Angel; Lavilla, Rodolfo; Vendrell, Marc
• Is Part Of: RSC chemical biology. Volume 3:Issue 10(2022)
• Page Start: 1251
• Page End: 1259
• Publication Date: 2022-09-01
• Subjects: 572
• DOI: 10.1039/d2cb00168c
• Languages: English
• ISSNs: 2633-0679

Abstract

Abstract : By combining synthetic multicomponent reactions and cell-based screenings, we identified NIRBD-62c as a multimodal probe for STED and fluorescence lifetime imaging of the vesicular traffic between intracellular compartments and the plasma membrane. Abstract : We report a platform combining multicomponent reaction synthesis and automated cell-based screening to develop biocompatible NIR-BODIPY fluorophores. From a library of over 60 fluorophores, we optimised compound NIRBD-62c as a multimodal probe with suitable properties for STED super-resolution and fluorescence lifetime imaging. Furthermore, we employed NIRBD-62c for imaging trafficking inside cells and to examine how pharmacological inhibitors can alter the vesicular traffic between intracellular compartments and the plasma membrane.