Glial expression of DmMANF is required for the regulation of activity, sleep and circadian rhythms in the visual system of Drosophila melanogaster. (17th March 2021)

• Record Type: Journal Article
• Authors: Walkowicz, Lucyna; Krzeptowski, Wojciech; Krzeptowska, Ewelina; Warzecha, Karolina; Sałek, Joanna; Górska‐Andrzejak, Jolanta; Pyza, Elżbieta
• Other Names: Majewska Ania guestEditor.; Verkhratsky Alexej guestEditor.; Hughes Ethan guestEditor.
• Is Part Of: European journal of neuroscience. Volume 54:Number 5(2021)
• Page Start: 5785
• Page End: 5797
• Publication Date: 2021-03-17
• Subjects: astrocyte‐like glia -- circadian clock -- ensheathing glia -- glial oscillators -- visual system; Nervous system -- Periodicals; 612.8
• DOI: 10.1111/ejn.15171
• Languages: English
• ISSNs: 0953-816X

Abstract

Abstract: DmMANF, Drosophila melanogaster mesencephalic astrocyte‐derived neurotrophic factor (DmMANF) is an evolutionarily conserved orthologue of mammalian MANF. This neurotrophic factor exerts many functions in the Drosophila brain, particularly those dependent on glial cells. As we found in our earlier study, downregulation of DmMANF in glia induces degeneration of glial cells in the first optic neuropil (lamina) where DmMANF abundance is especially high. In the present study, we observed that changes in the level of DmMANF in two types of glia, astrocyte‐like glia (AlGl) and ensheathing glia (EnGl), affect activity and sleep of flies. Interestingly, a proper level of DmMANF in AlGl seems to be important in guiding processes of pigment dispersing factor (PDF)‐expressing clock neurons. This is supported by our finding that DmMANF overexpression in AlGl leads to structural changes in the architecture of the PDF‐positive arborization in the brain. Finally, we detected that DmMANF also affects rhythms in glia themselves, as circadian oscillations in expression of the catalytic α subunit of the sodium pump in the lamina epithelial glia were abolished after DmMANF silencing. DmMANF expressed in AlGl and EnGl seems to affect the activity of neurons leading to changes in behaviour. Abstract : DmMANF‐level disturbances in AlGl and EnGl, caused by both overexpression and silencing, lead to their dysfunction and disorders in behaviour of Drosophila . Abnormal DmMANF level in AlGl results mainly in increasing the level of activity (in LD and DD) and in arborization changes of the clock neuron processes. In the case of EnGl, the changes are mainly observed in the decrease of day‐time and night‐time sleep.