Valorization of Waste Cooking Oil into Biodiesel via Bacillus stratosphericus Lipase Amine-Functionalized Mesoporous SBA-15 Nanobiocatalyst. (2nd November 2022)

• Record Type: Journal Article
• Authors: Ismail, Abdallah R.; Kashtoh, Hamdy; Betiha, Mohamed A.; Abu Amr, Salem A.; Baek, Kwang-Hyun; El-Gendy, Nour Sh.
• Other Names: Meshram Pratima Academic Editor.
• Is Part Of: International journal of chemical engineering. Volume 2022(2022)
• Publication Date: 2022-11-02
• Subjects: Chemical engineering -- Periodicals; Chemical engineering; Electronic journals; Periodicals; 660
• DOI: 10.1155/2022/7899996
• Languages: English
• ISSNs: 1687-806X

Abstract

Abstract : In this study, evaporation-induced self-assembly was applied to prepare amine-functionalized nano-silica (NH2 -Pr-SBA-15). That was simply used to immobilize Bacillus stratosphericus PSP8 lipase (E–NH2 –Pr-SBA-15), producing a nanobiocatalyst with good stability under vigorous shaking and a maximum lipase activity of 45 ± 2 U/mL. High-resolution X -ray diffractometer, Fourier transform infrared spectroscopy, N2 adsorption-desorption, field-emission scanning electron, and high-resolution transmission electron microscopic analyses proved the successful SBA-15 functionalization and enzyme immobilization. Response surface methodology based on a 1/2 fraction-three-levels face center composite design was applied to optimize the biodiesel transesterification process. This expressed efficient percentage conversion (97.85%) and biodiesel yield (97.01%) under relatively mild operating conditions: 3.12 : 1 methanol to oil ratio, 3.08 wt.% E–NH2 –Pr-SBA-15 loading, 48.6°C, 3.19 h at a mixing rate of 495.53 rpm. E–NH2 –Pr-SBA-15 proved to have a long lifetime, operational stability, and reusability.