Inside Back Cover: The Essential Role of Water Molecules in the Reaction Mechanism of Protein O‐Fucosyltransferase 2 (Angew. Chem. Int. Ed. 48/2022). Issue 48 (10th November 2022)

• Record Type: Journal Article
• Authors: Sanz‐Martínez, Ignacio; García‐García, Ana; Tejero, Tomás; Hurtado‐Guerrero, Ramón; Merino, Pedro
• Is Part Of: Angewandte Chemie international edition. Volume 61:Issue 48(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-11-10
• Subjects: Enzyme Catalysis -- Fucosylation -- Glycosyltransferases -- Protein Glycosylation -- QM/MM; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202216345
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract : Like a bow, PoFUT2 arms a peptide (arrow) and launches it once glycosylated with the help of a water environment. In their Research Article (e202213610), Ramón Hurtado‐Guerrero, Pedro Merino, and co‐workers show using QM/MM methods how the enzyme takes advantage of a high exposure of the active site, allowing water molecules to facilitate a pure SN 2 mechanism. The synergistic effect of an arginine residue and water molecules facilitates a smooth leaving of the pyrophosphate group.