Wastewater surveillance using ddPCR accurately tracked Omicron emergence due to altered N1 probe binding efficiency. Issue 10 (31st August 2022)

• Record Type: Journal Article
• Authors: Schussman, Melissa K.; Roguet, Adélaïde; Schmoldt, Angela; Dinan, Brooke; McLellan, Sandra L.
• Is Part Of: Environmental science. Volume 8:Issue 10(2022)
• Page Start: 2190
• Page End: 2195
• Publication Date: 2022-08-31
• Subjects: Water-supply -- Periodicals; Water security -- Periodicals; Water resources development -- Periodicals; Water chemistry -- Periodicals; 553.705
• DOI: 10.1039/d2ew00194b
• Languages: English
• ISSNs: 2053-1400

Abstract

Abstract : Wastewater surveillance for SARS-CoV-2 uses droplet digital (dd)PCR for absolute quantification and high sensitivity. We found ddPCR can also distinguish and accurately quantify Delta and Omicron variants due to a mutation in the probe binding site. Abstract : The emergence of Omicron in routine wastewater surveillance was accurately reflected using droplet digital (dd)PCR due to a mutation in the N1 probe binding region that caused diminished fluorescence within individual droplets. Wastewater from five cities provided a higher resolution of Omicron emergence when comparing data from sequenced clinical samples.