Enhanced calcium release at specialised surface sites compensates for reduced t-tubule density in neonatal sheep atrial myocytes. (December 2022)

• Record Type: Journal Article
• Authors: Smith, Charlotte E.R.; Pinali, Christian; Eisner, David A.; Trafford, Andrew W.; Dibb, Katharine M.
• Is Part Of: Journal of molecular and cellular cardiology. Volume 173(2022)
• Page Start: 61
• Page End: 70
• Publication Date: 2022-12
• Subjects: Atria -- Calcium -- T-tubule -- Sarcoplasmic reticulum -- Ryanodine receptor cluster; Cardiology -- Periodicals; Heart Diseases -- Periodicals; Molecular Biology -- Periodicals; Cardiologie -- Périodiques; Cardiology; Electronic journals; Periodicals; 616.12
• DOI: 10.1016/j.yjmcc.2022.08.360
• Languages: English
• ISSNs: 0022-2828

Abstract

Abstract: Cardiac myocytes rely on transverse (t)-tubules to facilitate a rapid rise in calcium throughout the cell. However, despite their importance in triggering synchronous Ca 2+ release, t-tubules are highly labile structures. They develop postnatally, increase in density during exercise training and are lost in diseases such as heart failure (HF). In the majority of settings, an absence of t-tubules decreases function. Here we show that despite reduced t-tubule density due to immature t-tubules, the newborn atrium is highly specialised to maintain Ca 2+ release. To compensate for fewer t-tubules triggering a central rise in Ca 2+, Ca 2+ release at sites on the cell surface is enhanced in the newborn, exceeding that at all Ca 2+ release sites in the adult. Using electron and super resolution microscopy to investigate myocyte ultrastructure, we found that newborn atrial cells had enlarged surface sarcoplasmic reticulum and larger, more closely spaced surface and central ryanodine receptor clusters. We suggest that these adaptations mediate enhanced Ca 2+ release at the sarcolemma and aid propagation to compensate for reduced t-tubule density in the neonatal atrium. Graphical abstract: Unlabelled Image Highlights: T-tubules are present at low density in newborn sheep atrial cells. In these cells, Ca 2+ release is enhanced at specialised sites on the cell surface. Ca 2+ release at these sites is greater than any location in the adult atrium. This may be explained by ultrastructural differences between newborn and adult. Newborn atrial cells have wider surface SR and larger, closer RyR clusters vs adult.