Leaves from four different sugarcane varieties as potential renewable feedstocks for second-generation ethanol production: Pretreatments, chemical composition, physical structure, and enzymatic hydrolysis yields. (October 2022)

• Record Type: Journal Article
• Authors: Espirito Santo, Melissa C.; Kane, Aissata Ousmane; Pellegrini, Vanessa O.A.; Thema, Force Tefo; García, José M.; Acevedo, Alberto; Erazzú, Luis E.; Guimaraes, Francisco E.G.; deAzevedo, Eduardo R.; Polikarpov, Igor
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 45(2022)
• Publication Date: 2022-10
• Subjects: Sugarcane leaves -- Sugarcane biotypes -- Physical structure -- Enzymatic hydrolysis -- Second-generation ethanol; ANOVA Analysis of variance -- CLSM Confocal Laser Scanning Microscopy -- CrI Crystallinity Index -- CPMAS Cross-Polarization Magic-Angle Spinning -- CPMG Carr-Purcell Meiboom-Gill -- EC Energy cane -- HPLC High-Performance Liquid Chromatography -- INTA National Institute of Agricultural Technology of Argentina -- NMR Nuclear Magnetic Resonance -- SC Sugar cane -- SCB Sugar Cane Bagasse -- SCL Sugar Cane Leaves -- SEM Scanning Electron Microscopy -- XRD X-ray diffraction; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2022.102485
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: Development of Bioeconomy is impossible without establishing efficient technologies of lignocellulosic biomass valorization via transformation into fermentable sugars. Sugarcane is a valuable agricultural crop in several Asian, African, and Latin American countries. Here, leaves from two sugarcane and two energy cane varieties were evaluated for sustainable production of second-generation sugars, and their enzymatic hydrolysis yields were compared. Structural, morphological, and chemical composition changes in sugar and energy cane leaves submitted to acid, and acid-alkaline pretreatments were analyzed using X-ray diffraction, scanning electron microscopy, confocal laser microscopy, high-performance liquid chromatography, and low-field solid-state NMR techniques. Enzymatic hydrolysis assays were conducted to evaluate saccharification yields of untreated and pretreated leaves. Jointly, our results revealed the significant potential of leaves from two commercial sugar cane cultivars currently bred in Argentina as possible lignocellulose substrates for the 2G ethanol industry. Highlights: Potential of leaves from four sugarcane varieties for 2 GE production was investigated. Impacts of pretreatments on the physical structure of the leaves were studied. Side-by-side comparison of enzymatic hydrolysis yields from four cultivars was done. The leaves from two commercial sugarcane cultivars are promising substrates for 2 GE.