Structural Model of a Porphyromonas gingivalis type IX Secretion System Shuttle Complex. Issue 23 (15th December 2022)

• Record Type: Journal Article
• Authors: Dorgan, Ben; Liu, Yichao; Wang, Sunjun; Aduse-Opoku, Joseph; Whittaker, Sara B.-M.; Roberts, Mark A.J.; Lorenz, Christian D.; Curtis, Michael A.; Garnett, James A.
• Is Part Of: Journal of molecular biology. Volume 434:Issue 23(2022)
• Publication Date: 2022-12-15
• Subjects: Porphyromonas gingivalis -- T9SS -- CTD -- RgpB -- PorV; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2022.167871
• Languages: English
• ISSNs: 0022-2836

Abstract

Graphical abstract: Highlights: How the T9SS interacts with its cargo proteins is not well understood. We present the structure/dynamics of a P. gingivalis secretion domain (RgpB-CTD). We model the structure/dynamics of the P. gingivalis T9SS component PorV alone and in complex with RgpB-CTD. This is the first structural insight into secretion domain recognition by the T9SS. Abstract: Porphyromonas gingivalis is a gram-negative oral anaerobic pathogen and is one of the key causative agents of periodontitis. P. gingivalis utilises a range of virulence factors, including the cysteine protease RgpB, to drive pathogenesis and these are exported and attached to the cell surface via the type IX secretion system (T9SS). All cargo proteins possess a conserved C-terminal signal domain (CTD) which is recognised by the T9SS, and the outer membrane β-barrel protein PorV (PG0027/LptO) can interact with cargo proteins as they are exported to the bacterial surface. Using a combination of solution nuclear magnetic resonance (NMR) spectroscopy, biochemical analyses, machine-learning-based modelling and molecular dynamics (MD) simulations, we present a structural model of a PorV:RgpB-CTD complex from P. gingivalis . This is the first structural insight into CTD recognition by the T9SS and shows how the conserved motifs in the CTD are the primary sites that mediate binding. In PorV, interactions with extracellular surface loops are important for binding the CTD, and together these appear to cradle and lock RgpB-CTD in place. This work provides insight into cargo recognition by PorV but may also have important implications for understanding other aspects of type-IX dependent secretion.