Growth Differentiation Factor 7 promotes multiple-lineage differentiation in tenogenic cultures of mesenchymal stem cells. Issue 12 (December 2022)

• Record Type: Journal Article
• Authors: Kumlin, Maritha; Lindberg, Karolina; Haldosen, Lars-Arne; Felländer-Tsai, Li; Li, Yan
• Is Part Of: Injury. Volume 53:Issue 12(2022)
• Page Start: 4165
• Page End: 4168
• Publication Date: 2022-12
• Subjects: Tendon regeneration -- Tenocyte -- Osteoblast -- Adipocyte -- Growth Differentiation Factor 7 (GDF-7) -- Mesenchymal stem cells (MSCs); Wounds and injuries -- Surgery -- Periodicals; Accidents -- Periodicals; Wounds and Injuries -- surgery -- Periodicals; Lésions et blessures -- Chirurgie -- Périodiques; Electronic journals; Electronic journals; 617.1
• DOI: 10.1016/j.injury.2022.09.017
• Languages: English
• ISSNs: 0020-1383

Abstract

Highlights: This study describes an in vitro model with murine stem cells (C3H10) for the study of tenogenic differentiation. Growth differentiation factor 7 (GDF-7) stimulates tenogenic differentiation in this model. GDF-7 also stimulates adipogenic and osteogenic differentiation. Abstract: The repair of the tendon-bone interface, which is composed of tendon, fibrocartilage, and bony attachment, remains a clinical challenge. The application of mesenchymal stem cells (MSCs), collagen-rich extracellular matrix (ECMs), as well as growth factors, has the potential to regenerate this special multiple-tissue structure through the so-called biological augmentation. We present here an in vitro tendon regeneration model with C3H10T1/2 cells cultured on Collagen I matrix and evaluated the lineage determination effects of Growth Differentiation Factor 7 (GDF-7). We found that besides tenogenic effect, GDF-7 also stimulates the expression of osteoblastic as well as adipocytic genes. Our results indicate that GDF-7 might be a promising growth factor for regeneration of the tendon-bone interface due to its multiple-lineage stimulating effects. However, the side effect on adipogenic differentiation should be of concern, as it is a known risk factor for repair failures.