Identification of two theranostic biomarker panels for epithelial ovarian cancer. (January 2023)

• Record Type: Journal Article
• Authors: Habel, Azza; Xu, Weili; Hadj Ahmed, Mariem; Stayoussef, Mouna; Bouaziz, Hanen; Ayadi, Mouna; Mezlini, Amel; Larbi, Anis; Yaacoubi-Loueslati, Basma
• Is Part Of: Cytokine. Volume 161(2023)
• Publication Date: 2023-01
• Subjects: Epithelial ovarian cancer -- Luminex -- Microenvironement -- Cytokines -- resistance to CT -- Theranostic biomarkers; Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2022.156051
• Languages: English
• ISSNs: 1043-4666

Abstract

Graphical abstract: Highlights: Tumor microenvironment is involved in the development of Epithelial ovarian cancer. Potential involvement of the tumor microenvironment therapeutically to prevent the development of chemoresistance. A panel of 7 analytes (IL-9, TNF-α, Eotaxin, IP-10, BLC, Fractalkine and Tweak) that may represent potential biomarkers for early diagnosis of EOC. A panel of five cytokines (INF-γ, IL-1β, IL-8, MIP-1β, TNF-α) that may represent potential biomarkers for high risk of developing resistance to the basic therapy of EOC. Abstract: Background: Epithelial Ovarian cancer (EOC) is the leading cause of death associated with gynecologic tumors. Because the disease is asymptomatic in early-stage, the majority of patients are not diagnosed until late stages, highlighting the need for the development of novel diagnostic biomarkers. Mediators of tumoral microenvironment may affect EOC progression and resistance to treatment. Aim of the study: Analysis of serum proteins to identify a panel of theranostic biomarkers for EOC. Patients and methods: Serum levels of 65 analytes were determined in EOC patients, and healthy controls with the ProcartaPlex Human Immune Monitoring 65-Plex Panel. Results: Twenty-one analytes: 7 cytokines (IFN-γ, IL-12p70, IL-13, IL-18 and TSLP), 7 chemokines (Eotaxin, eotaxin-2, IP-10, BLC, I-TAC, SDF-1α, and fractalkine), 2 growth factors (MMP-1, VEGF-α), and 5 soluble receptors (APRIL, CD40L, TWEAK, CD30 and TNFRII; were significantly differentially expressed between the two groups. ROC curves showed that only seven of them (IL-9, TNF-α, Eotaxin, IP-10, BLC, Fractalkine, and Tweak) had AUC values greater than 0.70 and thus had potential clinical utility. Moreover, five cytokines: IFN-γ, IL-1 β, IL-8, MIP-1β, and TNF-α are positively associated with patients who developed resistance to taxol-platinum-based chemotherapy (CT). Conclusion: This study has revealed a first panel of 7 analytes (IL-9, TNF-α, Eotaxin, IP-10, BLC, Fractalkine and Tweak) that can be used for early detection of EOC and a second panel of five cytokines (IFN-γ, IL-1β, IL-8, MIP-1β, TNF-α) that can help clinicians to identify EOC patients who are at higher risk to develop resistance to CT of EOC.