Bioaugmentation reconstructed nitrogen metabolism in full-scale simultaneous partial nitrification-denitrification, anammox and sulfur-dependent nitrite/nitrate reduction (SPAS). (January 2023)

• Record Type: Journal Article
• Authors: Liu, Huimin; Liu, Dejin; Huang, Zhenyu; Chen, Yuancai
• Is Part Of: Bioresource technology. Volume 367(2023)
• Publication Date: 2023-01
• Subjects: Simultaneous nitrification and denitrification -- Sulfur-dependent nitrite/nitrate reduction -- Fermentation pharmaceutical wastewater -- Two-stage A/O -- Bioaugmentation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.128233
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: A full-scale SPAS process was established by inoculating with BC-SND and BC-SOB. More than 93 % TN and 98 % NH4 + -N removal could be achieved in the first A/O stage. S 0 -SAD could coordinate with anammox to exert further deep denitrification. SPAS process was a synergistic pathway for nitrogen removal. Bioaugmentation significantly up-regulated genes related to microbial metabolism. Abstract: To enhance nitrogen removal of fermentation pharmaceutical wastewater with high nitrogen load, a full-scale process based on simultaneous partial nitrification-denitrification/ anammox/ sulfur autotrophic denitrification (SPAS) was established via inoculating with bioaugmentation consortia in a modified two-stage AO. More than 93 % TN and 98 % NH4 + -N removal were obtained at a rate of 0.8 kg-N/ m 3 /d in the first A/O stage, in which short-cut SND was involved with 96.05 % ESND when bioaugmented with SND, while S 0 -SAD could coordinate with anammox to exert further deep denitrification in the second A/O stage. KEGG analysis demonstrated that the SPAS process was synergism of HD, PN/PDN, SND, SAD and anammox metabolism, bioaugmentation could significantly up-regulate genes related to microbial metabolism (TCA cycle, Carbon metabolism, ABC transporters) and environmental adaptation (Two-component system, Quorum sensing) based on the FAPROTAX and Picrust2 functional prediction. This study provided a new perspective in engineering applications.