Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65. (1st December 2022)

• Record Type: Journal Article
• Authors: Tan, Jingwen; Sun, Mingjun; Yin, Jinyao; Zhou, Qian; Zhao, Ruihuan; Chen, Qian; Sun, Huiwen; Jiang, Chenglan; Li, Shuting; He, Yuefeng
• Is Part Of: Chemico-biological interactions. Volume 368(2022)
• Publication Date: 2022-12-01
• Subjects: Arsenic -- NF-κB and P53 balance -- hsa_circ_0005050 -- circRNA-protein complex -- Cell apoptosis -- Cell proliferation; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2022.110208
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: The regulatory network between arsenic, genes and signaling pathways has been reported in arsenic carcinogenesis. Studies on circRNA represent a growing field, but the extent to circRNA potential mechanisms remains poorly understood. So this study we explore the systematic function of hsa_circ_0005050 in mediating the cell apoptosis and proliferation. We demonstrated that hsa_circ_0005050 was highly expressed in subjects who are long-term exposed to arsenic, and could be induced by NaAs2 O3 in A549 and 16HBE. Knockdown of hsa_circ_0005050 promotes A549 cell viability, whereas exerts the opposite effects in 16HBE. Mechanistically, hsa_circ_0005050 regulates the p53 and NF-κB signaling pathway involved in the apoptosis and proliferation. And we found that hsa_circ_0005050 could directly bind to the RNA binding protein ILF3 and mutually influence each other's formation. Upon si-hsa_circ_0005050, ILF3 export to the cytoplasm resulting the formation of a ternary complex ILF3-p65-IκBA, breaks the balance of p53 and NF-κB pathway and induces A549 apoptosis and leads to 16HBE proliferation. As a result of these investigations, suggestions were identified for future research. Highlights: Arsenic exposure induced hsa_circ_0005050 expression in vivo and vitro. Si-hsa_circ_0005050 cause an opposite effect on cell apoptosis and proliferation. Si-hsa_circ_0005050 leads to the disruption of the balance between p53 and p65. Si-hsa_circ_0005050 promote ILF3 exporting and forming the complex ILF3-p65-IκBA.