The pre-addition of "blocking" proteins decreases subsequent cellulase adsorption to lignin and enhances cellulose hydrolysis. (January 2023)

• Record Type: Journal Article
• Authors: Liu, Jingyun; Wu, Jie; Lu, Yi; Zhang, Huaiyu; Hua, Qi; Bi, Ran; Rojas, Orlando; Renneckar, Scott; Fan, Senqing; Xiao, Zeyi; Saddler, Jack
• Is Part Of: Bioresource technology. Volume 367(2023)
• Publication Date: 2023-01
• Subjects: Cellulase -- Lignin -- Non-productive binding -- Non-catalytic proteins -- QCM-D -- Langmuir adsorption; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.128276
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphic abstract: Highlights: Layer-by-layer QCM-D adsorption of non-catalytic proteins and enzymes to lignin. Kinetic analysis of enzyme and non-catalytic protein adsorption to lignin. Reduced rate, amount of enzyme adsorbed to lignin and binding after blocking. Abstract: The pre-adsorption of non-catalytic/blocking proteins onto the lignin component of pretreated biomass has been shown to significantly increase the effectiveness of subsequent enzyme-mediated hydrolysis of the cellulose by limiting non-productive enzyme adsorption. Layer-by-layer adsorption of non-catalytic proteins and enzymes onto lignin was monitored using Quartz Crystal Micro balancing combined with Dissipation monitoring (QCM-D) and conventional protein adsorption. These methods were used to assess the interaction between soft/hardwood lignins, cellulases and the three non-catalytic proteins BSA, lysozyme and ovalbumin. The QCM-D analysis showed higher adsorption rates for all of the non-catalytic proteins onto the lignin films as compared to cellulases. This suggested that the "blocking" proteins would preferentially adsorb to the lignin rather than the enzymes. Pre-incubation of the lignin films with blocking proteins resulted in reduced adsorption of cellulases onto the lignin, significantly enhancing cellulose hydrolysis.