Immunogenicity of engineered probiotics expressing conserved antigens of influenza virus and FLIC flagellin against H9N2 AIVinfection in mice. (31st December 2022)

• Record Type: Journal Article
• Authors: Hu, Tian-Yang; Lian, Yi-Bing; Qian, Jia-Hao; Yang, Yong-Lei; Ata, Emad Beshir; Zhang, Rong-Rong; Shi, Chun-Wei; Yang, Gui-Lian; Huang, Hai-Bin; Jiang, Yan-Long; Wang, Jian-Zhong; Cao, Xin; NanWang, ; Zeng, Yan; Yang, Wen-Tao; Wang, Chun-Feng
• Is Part Of: Research in veterinary science. Volume 153(2022)
• Page Start: 115
• Page End: 126
• Publication Date: 2022-12-31
• Subjects: Lactobacillus plantarum -- Influenza virus -- FLIC flagellin -- Mucosal immunity; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2022.10.024
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Avian influenza virus (AIV)is easy to cause diseases in birds and humans.It causes great economic losses to the poultry farms and leads to public health problems. Using vaccines is the main approach to control the prevalence of AIV. In our previously published article, a recombinant Lactobacillus plantarum ( L. plantarum ) expressing the NP-M2 peptide ofH9N2 AIV was generated, and its protective effect was evaluated in a chicken model. In this study, the protective effect was estimated in mice model. Humoral and cellular immune response parameters were measured using flow cytometry adding to body weight loss, survival rate, virus load, and histopathological changes in the lung. The obtained results elucidated that, the recombinant L. plantarum can promote the activation of dendritic cells (DC), proliferation of T and B cells adding to eliciting protective secretory IgA (sIgA) and humeral IgG level in mice model. Accordingly, it could be used as a patent vaccine to control the AIV infection. Highlights: Activation of CD80 and CD86 molecules on DCs by N/pgsA'-NP-M2-FLIC. TNF+, IL-2+ and IFN-γ + producing CD8+ T cells in the MLN and spleen were induced by the N/pgsA'-NP-M2-FLIC in mice. N/pgsA'-NP-M2-FLIC provided 80% protection against the mouse-adapted H9N2 virus and reduced virus replication in the lungs after challenge.