Protease‐ and cell type–specific activation of protease‐activated receptor 2 in cutaneous inflammation. (12th October 2022)

• Record Type: Journal Article
• Authors: Fleischer, Maria Isabel; Röhrig, Nadine; Raker, Verena K.; Springer, Juliane; Becker, Detlef; Ritz, Sandra; Bros, Matthias; Stege, Henner; Haist, Maximilian; Grabbe, Stephan; Haub, Jessica; Becker, Christian; Reyda, Sabine; Disse, Jennifer; Schmidt, Talkea; Mahnke, Karsten; Weiler, Hartmut; Ruf, Wolfram; Steinbrink, Kerstin
• Is Part Of: Journal of thrombosis and haemostasis. Volume 20:Number 12(2022)
• Page Start: 2823
• Page End: 2836
• Publication Date: 2022-10-12
• Subjects: allergic contact dermatitis -- contact hypersensitivity -- factor Xa -- protease‐activated receptor 2 -- tissue factor; Thrombosis -- Periodicals; Hemostasis -- Periodicals; Blood coagulation disorders -- Periodicals; 616.1
• DOI: 10.1111/jth.15894
• Languages: English
• ISSNs: 1538-7933

Abstract

Abstract: Background: Protease‐activated receptor 2 (PAR2) signaling controls skin barrier function and inflammation, but the roles of immune cells and PAR2‐activating proteases in cutaneous diseases are poorly understood. Objective: To dissect PAR2 signaling contributions to skin inflammation with new genetic and pharmacological tools. Methods/Results: We found markedly increased numbers of PAR2 + infiltrating myeloid cells in skin lesions of allergic contact dermatitis (ACD) patients and in the skin of contact hypersensitivity (CHS) in mice, a murine ACD model for T cell–mediated allergic skin inflammation. Cell type–specific deletion of PAR2 in myeloid immune cells as well as mutation‐induced complete PAR2 cleavage insensitivity significantly reduced skin inflammation and hapten‐specific Tc1/Th1 cell response. Pharmacological approaches identified individual proteases involved in PAR2 cleavage and demonstrated a pivotal role of tissue factor (TF) and coagulation factor Xa (FXa) as upstream activators of PAR2 in both the induction and effector phase of CHS. PAR2 mutant mouse strains with differential cleavage sensitivity for FXa versus skin epithelial cell–expressed proteases furthermore uncovered a time‐dependent regulation of CHS development with an important function of FXa‐induced PAR2 activation during the late phase of skin inflammation. Conclusions: Myeloid cells and the TF–FXa–PAR2 axis are key mediators and potential therapeutic targets in inflammatory skin diseases. Abstract : Myeloid cells infiltrate the skin in contact hypersensitivity (CHS) and promote skin inflammation through protease activated receptor (PAR) 2 signaling. PAR2 mutants with resistance to selective proteases implicate coagulation FXa as a driver for the persistence of inflammation after allergen challenge. Pharmacological inhibitions indicate that the TF‐FXa‐PAR2 axis is a potential therapeutic target in CHS.