Determination of B Vitamins by Double-Vortex-Ultrasonic Assisted Dispersive Liquid–Liquid Microextraction and Evaluation of their Possible Roles in Susceptibility to COVID−19 Infection: Hybrid Box–Behnken Design and Genetic Algorithm. (27th October 2021)

• Record Type: Journal Article
• Authors: Akbari, Akbar; Koolivand, Zahra; Mohamadi, Mohamad Bagher; Zahedi, Atefeh; Ghasemnezhad, Masoumeh; Sabati, Zolfaghar; Chamkouri, Narges
• Is Part Of: Journal of chromatographic science. Volume 60:Number 9(2022)
• Page Start: 897
• Page End: 906
• Publication Date: 2021-10-27
• Subjects: Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1093/chromsci/bmab124
• Languages: English
• ISSNs: 0021-9665

Abstract

Abstract: Objectives: In this study, double-vortex-ultrasonic assisted dispersive liquid–liquid microextraction (DVUDLLME) was applied to determine the concentration of vitamin B9, 5-methyl tetrahydrofolate (5-MeTHF) and vitamin B12 in human serum samples. Methods: High-performance liquid chromatography (HPLC) coupled with DVUDLLME was applied to analyze vitamins B in patients with Coronavirus disease (COVID-19). Then, significant variables were chosen and optimized using the hybrid Box–Behnken design and genetic algorithm. Results: The detection limits of DVUDLLME–HPLC were 0.21 ng mL −1, 0.18 ng mL −1 and 55 pgmL −1 for vitamin B9, 5-MeTHF and vitamin B12, respectively. Subsequently, DVUDLLME-HPLC was applied to measure B vitamins and investigated their possible roles in susceptibility to COVID-19 infection. Fifty-seven percent of the patients without an underlying disease have significantly lower serum vitamin B12 levels in comparison to controls. Conclusions: The advantages of this method are low detection limit, simple preparation, low retention time and the use of a cheaper technique instead of expensive mass detectors. The results suggest that vitamin B12 deficiency may decrease the immune system defenses against COVID-19 patients without an underlying disease and cause the disease to become severe. However, these works need a large population and further research, such as a randomized trial and a cohort study.