Membrane‐type frizzled‐related protein regulates lipidome and transcription for photoreceptor function. Issue 1 (28th November 2019)

• Record Type: Journal Article
• Authors: Kautzmann, Marie‐Audrey I.; Gordon, William C.; Jun, Bokkyoo; Do, Khanh V.; Matherne, Blake J.; Fang, Zhide; Bazan, Nicolas G.
• Is Part Of: FASEB journal. Volume 34:Issue 1(2020)
• Page Start: 912
• Page End: 929
• Publication Date: 2019-11-28
• Subjects: Adipor1 -- inflammatory signaling -- matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) -- retinal degenerations -- RPE cell -- VLC‐PUFAs; Biology -- Periodicals; Biology, Experimental -- Periodicals; 570
• DOI: 10.1096/fj.201902359R
• Languages: English
• ISSNs: 0892-6638

Abstract

Abstract: Molecular decision‐makers of photoreceptor (PRC) membrane organization and gene regulation are critical to understanding sight and retinal degenerations that lead to blindness. Using Mfrp rd6 mice, which develop PRC degeneration, we uncovered that membrane‐type frizzled‐related protein (MFRP) participates in docosahexaenoic acid (DHA, 22:6) enrichment in a manner similar to adiponectin receptor 1 (AdipoR1). Untargeted imaging mass spectrometry demonstrates cell‐specific reduction of phospholipids containing 22:6 and very long‐chain polyunsaturated fatty acids (VLC‐PUFAs) in Adipor1 −/− and Mfrp rd6 retinas. Gene expression of pro‐inflammatory signaling pathways is increased and gene‐encoding proteins for PRC function decrease in both mutants. Thus, we propose that both proteins are necessary for retinal lipidome membrane organization, visual function, and to the understanding of the early pathology of retinal degenerative diseases.