A novel F‐box domain containing cyclin F like gene is required for maintaining the genome stability and survival of chicken primordial germ cells. Issue 1 (28th November 2019)

• Record Type: Journal Article
• Authors: Lee, Bo Ram; Rengaraj, Deivendran; Choi, Hee Jung; Han, Jae Yong
• Is Part Of: FASEB journal. Volume 34:Issue 1(2020)
• Page Start: 1001
• Page End: 1017
• Publication Date: 2019-11-28
• Subjects: gene expression -- gene identification -- gene regulation; Biology -- Periodicals; Biology, Experimental -- Periodicals; 570
• DOI: 10.1096/fj.201901294R
• Languages: English
• ISSNs: 0892-6638

Abstract

Abstract: The stability and survival of germ cells are controlled by the germline‐specific genes, however, such genes are less known in the avian species. Using a microarray‐based the National Center for Biotechnology Information Gene Expression Omnibus dataset, we found an unigene (Gga.9721) that upregulated in the chicken primordial germ cells (PGCs). The unigene showed 97% identities with an uncharacterized chicken cyclin F like gene. The predicted chicken cyclin F like gene was further characterized through expression and regulation in the chicken PGCs. The sequence analysis revealed that the gene shows identities with cyclin F gene and contains an F‐box domain. The expression of chicken cyclin F like was detected specifically in the gonads, PGCs, and germline cells. The knockdown of cyclin F like gene resulted in DNA damage and apoptosis in the PGCs. The genes related to stemness and germness were downregulated, whereas, genes related to apoptosis and DNA damage response were increased in the PGCs after the knockdown of chicken cyclin F like. We further observed that the Nanog homeobox controlled the transcriptional activity of chicken cyclin F like gene in PGCs. Collectively, the chicken cyclin F like gene, which is not reported in any other species, is required for maintaining the genome stability of germ cells.