Tumor necrosis factor‐induced ArhGEF10 selectively activates RhoB contributing to human microvascular endothelial cell tight junction disruption. Issue 6 (4th May 2021)

• Record Type: Journal Article
• Authors: Khan, Alamzeb; Ni, Weiming; Lopez‐Giraldez, Francesc; Kluger, Martin S.; Pober, Jordan S.; Pierce, Richard W.
• Is Part Of: FASEB journal. Volume 35:Issue 6(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-05-04
• Subjects: capillary leak -- inflammation -- permeability -- Rho guanine nucleotide‐exchange factor -- transendothelial electrical resistance -- vascular dysfunction; Biology -- Periodicals; Biology, Experimental -- Periodicals; 570
• DOI: 10.1096/fj.202002783RR
• Languages: English
• ISSNs: 0892-6638

Abstract

Abstract: Capillary endothelial cells (ECs) maintain a semi‐permeable barrier between the blood and tissue by forming inter‐EC tight junctions (TJs), regulating selective transport of fluid and solutes. Overwhelming inflammation, as occurs in sepsis, disrupts these TJs, leading to leakage of fluid, proteins, and small molecules into the tissues. Mechanistically, disruption of capillary barrier function is mediated by small Rho‐GTPases, such as RhoA, ‐B, and ‐C, which are activated by guanine nucleotide exchange factors (GEFs) and disrupted by GTPase‐activating factors (GAPs). We previously reported that a mutation in a specific RhoB GAP (p190BRhoGAP) underlays a hereditary capillary leak syndrome. Tumor necrosis factor (TNF) treatment disrupts TJs in cultured human microvascular ECs, a model of capillary leak. This response requires new gene transcription and involves increased RhoB activation. However, the specific GEF that activates RhoB in capillary ECs remains unknown. Transcriptional profiling of cultured tight junction‐forming human dermal microvascular endothelial cells (HDMECs) revealed that 17 GEFs were significantly induced by TNF. The function of each candidate GEF was assessed by short interfering RNA depletion and trans‐endothelial electrical resistance screening. Knockown of ArhGEF10 reduced the TNF‐induced loss of barrier which was phenocopied by RhoB or dual ArhGEF10/RhoB knockdown. ArhGEF10 knockdown also reduced the extent of TNF‐induced RhoB activation and disruption at tight junctions. In a cell‐free assay, immunoisolated ArhGEF10 selectively catalyzed nucleotide exchange to activate RhoB, but not RhoA or RhoC. We conclude ArhGEF10 is a TNF‐induced RhoB‐selective GEF that mediates TJ disruption and barrier loss in human capillary endothelial cells.