Binding of Water‐Soluble CdSe Quantum Dots with Human Serum Albumin: Further Studies into their Effects on Dietary Polyphenol Binding and Sensing of Antibiotic Lomefloxacin. Issue 40 (26th October 2021)

• Record Type: Journal Article
• Authors: Haque, Mahabul; Santra, Sujan; Paul, Debojit; Roy, Atanu Singha
• Is Part Of: ChemistrySelect. Volume 6:Issue 40(2021)
• Page Start: 11144
• Page End: 11156
• Publication Date: 2021-10-26
• Subjects: Binding constant -- CdSe Quantum Dots; Fluorescence -- Human Serum Albumin (HSA) -- Lomefloxacin.; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.202102212
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Synthesis of surface modified quantum dots (QDs) having potential biological activities are great important in biomedicinal chemistry. Here, we have synthesized mercapto propanoic acid (MPA) and thiolactic acid (TLA) coated water soluble cadmium selenide (CdSe) QDs having biological activity. Dispersion of QDs into a biological fluid can form protein corona. Hence, the binding of CdSe QDs with the carrier protein, human serum albumin (HSA) have been executed using spectroscopic methods. The binding affinity order of CdSe QDs‐HSA complexes were found in the order of 10 5 M −1 at 298 K. CdSe QDs are also affects the binding affinities of dietary polyphenols (naringin: NG and naringenin: NRG) towards HSA. The synthesized CdSe QDs was employed to sensing of antibiotic lomefloxacin by fluorescence quenching mechanism. The detection limit was found 0.70‐1.83 μg mL −1 . The CdSe QDs also demonstrate the detection of lomefloxacin under uv light based on color change of the solutions. Abstract : The synthesis and characterization of mercapto propanoic acid/thiolactic acid (MPA/TLA) capped cadmium selenide quantum dots (CdSe QDs) were carried out using differential spectroscopic techniques. Further we have investigated their binding properties with the carrier protein human serum albumin (HSA) using spectroscopic methods. The sensing study of antibiotic lomefloxacin (LMF) by these QDs were also conducted using fluorescence spectroscopy and under UV‐light (naked eye) detection.