P62‐positive aggregates are homogenously distributed in the myocardium and associated with the type of mutation in genetic cardiomyopathy. Issue 6 (18th February 2021)

• Record Type: Journal Article
• Authors: van der Klooster, Zoë Joy; Sepehrkhouy, Shahrzad; Dooijes, Dennis; te Rijdt, Wouter P.; Schuiringa, Frederique S. A. M.; Lingeman, Jolanthe; van Tintelen, Johannes Peter; Harakalova, Magdalena; Goldschmeding, Roel; Suurmeijer, Albert J. H.; Asselbergs, Folkert W.; Vink, Aryan
• Is Part Of: Journal of cellular and molecular medicine. Volume 25:Issue 6(2021)
• Page Start: 3160
• Page End: 3166
• Publication Date: 2021-02-18
• Subjects: autophagy -- cardiomyopathy -- desminopathy -- genetic -- histology -- P62 -- pathology -- phospholamban -- senescence -- sequestosome‐1; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.16388
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: Genetic cardiomyopathy is caused by mutations in various genes. The accumulation of potentially proteotoxic mutant protein aggregates due to insufficient autophagy is a possible mechanism of disease development. The objective of this study was to investigate the distribution in the myocardium of such aggregates in relation to specific pathogenic genetic mutations in cardiomyopathy hearts. Hearts from 32 genetic cardiomyopathy patients, 4 non‐genetic cardiomyopathy patients and 5 controls were studied. Microscopic slices from an entire midventricular heart slice were stained for p62 (sequestosome‐1, marker for aggregated proteins destined for autophagy). The percentage of cardiomyocytes with p62 accumulation was higher in cardiomyopathy hearts (median 3.3%) than in healthy controls (0.3%; P < .0001). p62 accumulation was highest in the desmin (15.6%) and phospholamban (7.2%) groups. P62 accumulation was homogeneously distributed in the myocardium. Fibrosis was not associated with p62 accumulation in subgroup analysis of phospholamban hearts. In conclusion, accumulation of p62‐positive protein aggregates is homogeneously distributed in the myocardium independently of fibrosis distribution and associated with desmin and phospholamban cardiomyopathy. Proteotoxic protein accumulation is a diffuse process in the myocardium while a more localized second hit, such as local strain during exercise, might determine whether this leads to regional myocyte decay.