Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea. Issue 3 (5th September 2022)

• Record Type: Journal Article
• Authors: Wang, Yu‐Qin; Li, Guang‐Ya; Li, Lu; Song, Qi‐Sheng; Stanley, David; Wei, Shu‐Jun; Zhu, Jia‐Ying
• Other Names: Zhu Jia‐Ying guestEditor.; Song Qisheng guestEditor.; Stanley David guestEditor.
• Is Part Of: Archives of insect biochemistry and physiology. Volume 111:Issue 3(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-09-05
• Subjects: cytochrome P450 monooxygenase -- expression profiling -- genomics -- molecular evolution -- Tenebrio molitor; Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21954
• Languages: English
• ISSNs: 0739-4462

Abstract

Abstract: Cytochrome P450 monooxygenases (CYPs) are present in almost all areas of the tree of life. As one of the largest and most diverse superfamilies of multifunctional enzymes, they play important roles in the metabolism of xenobiotics and biosynthesis of endogenous compounds, shaping the success of insects. In this study, the CYPome (an omics term for all the CYP genes in a genome) diversification was examined in the four Tenebrionidea species through genome‐wide analysis. A total of 483 CYP genes were identified, of which 103, 157, 122, and 101 were respectively deciphered from the genomes of Tebebrio molitor, Asbolus verucosus, Hycleus cichorii and Hycleus phaleratus . These CYPs were classified into four major clans (mitochondrial, CYP2, CYP3, and CYP4), and clans CYP3 and CYP4 are most diverse. Phylogenetic analysis showed that most CYPs of these Tenebrionidea beetles from each clan had a very close 1:1 orthology to each other, suggesting that they originate closely and have evolutionally conserved function. Expression analysis at different developmental stages and in various tissues showed the life stage‐, gut‐, salivary gland‐, fat body‐, Malpighian tubule‐, antennae‐, ovary‐ and testis‐specific expression patterns of T. molitor CYP genes, implying their various potential roles in development, detoxification, immune response, digestion, olfaction, and reproduction. Our studies provide a platform to understand the evolution of Tenebrionidea CYP gene superfamily, and a basis for further functional investigation of the T. molitor CYPs involved in various biological processes. Abstract : CYP genes in the genome of five tenebrionids. Phylogenetic and gene expression profiling analyses provide clues for their functions. Highlights: Four hundred eighty three CYP genes were identified in the genomes of four tenebrionids. Gene family evolution of CYPs was comparatively analyzed in three darkling and two meloid beetles. Spatial and temporal expression of CYP genes were profiled in selected developmental stages and tissues of the yellow mealworm, Tenebrio molior . Our findings provide a resource for investigating CYPs in yellow mealworm.