3‐Chloro‐5‐Substituted‐1, 2, 4‐Thiadiazoles (TDZs) as Selective and Efficient Protein Thiol Modifiers. (27th September 2022)

• Record Type: Journal Article
• Authors: Jänsch, Niklas; Frühauf, Anton; Schweipert, Markus; Debarnot, Cécile; Erhardt, Miriam; Brenner‐Weiss, Gerald; Kirschhöfer, Frank; Jasionis, Tomas; Čapkauskaitė, Edita; Zubrienė, Asta; Matulis, Daumantas; Meyer‐Almes, Franz‐Josef
• Is Part Of: Chembiochem. Volume 23:Number 21(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-09-27
• Subjects: biotin switch assay -- covalent inactivators -- nucleophilic aromatic substitution -- proteomic studies -- thiol modification; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202200417
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: The study of cysteine modifications has gained much attention in recent years. This includes detailed investigations in the field of redox biology with focus on numerous redox derivatives like nitrosothiols, sulfenic acids, sulfinic acids and sulfonic acids resulting from increasing oxidation, S‐lipidation, and perthiols. For these studies selective and rapid blocking of free protein thiols is required to prevent disulfide rearrangement. In our attempt to find new inhibitors of human histone deacetylase 8 (HDAC8) we discovered 5‐sulfonyl and 5‐sulfinyl substituted 1, 2, 4‐thiadiazoles (TDZ), which surprisingly show an outstanding reactivity against thiols in aqueous solution. Encouraged by these observations we investigated the mechanism of action in detail and show that these compounds react more specifically and faster than commonly used N ‐ethyl maleimide, making them superior alternatives for efficient blocking of free thiols in proteins. We show that 5‐sulfonyl‐TDZ can be readily applied in commonly used biotin switch assays. Using the example of human HDAC8, we demonstrate that cysteine modification by a 5‐sulfonyl‐TDZ is easily measurable using quantitative HPLC/ESI‐QTOF‐MS/MS, and allows for the simultaneous measurement of the modification kinetics of seven solvent‐accessible cysteines in HDAC8. Abstract : 5‐Sulfonyl and 5‐sulfinyl substituted 1, 2, 4‐thiadiazoles (TDZ) are very rapid and selective modifiers of solvent‐accessible free‐thiol groups on proteins. TDZs can be used in ESI‐MS/MS studies or conventional biotin‐switch assays to analyze fast oxidation processes of distinct cysteines in a protein.