End-condition for solution small angle X-ray scattering measurements by kernel density estimation. Issue 1 (31st December 2022)

• Record Type: Journal Article
• Authors: Sekiguchi, Hiroshi; Ohta, Noboru; Ishibashi, Hideaki; Hino, Hideitsu; Mizumaki, Masaichiro
• Is Part Of: Science and Technology of Advanced Materials: Methods. Volume 2:Issue 1(2022)
• Page Start: 426
• Page End: 434
• Publication Date: 2022-12-31
• Subjects: SAXS -- Bio-SAXS -- optimum exposures -- kernel density estimation
• DOI: 10.1080/27660400.2022.2140021
• Languages: English
• ISSNs: 2766-0400

Abstract

ABSTRACT: We develop a method for calculating the minimum X-ray exposure time for X-ray solution scattering experiments using statistical and mathematical approaches to enhance the measurement efficiency while maintaining data quality. Experts can determine the X-ray exposure time for samples by investigating time-series data of scattering profiles and considering the quality of scattering profiles and the samples' X-ray damage. In this study, we apply a statistical inequality to estimate the kernel density estimation method's error to determine the minimum X-ray exposure time for protein solution scattering experiments and evaluate the method's validity using the results of Guinier analysis as an indicator. GRAPHICAL ABSTRACT: uf0001