A simple system for extracting water from soil and plant substrates –effects of substrate-derived exchangeable hydrogen on isotope ratios of extracted water–. Issue 6 (2nd November 2022)

• Record Type: Journal Article
• Authors: Ayabe, Yoshiko; Kakiuchi, Hideki; Watanabe, Hiroshi; Fujii, Masanori; Nagai, Masaru; Tani, Takashi
• Is Part Of: Journal of forest research. Volume 27:Issue 6(2022)
• Page Start: 429
• Page End: 438
• Publication Date: 2022-11-02
• Subjects: Cryogenic vacuum distillation -- isotope analysis -- isotope fractionation effect -- exchangeable hydrogen -- soil hydrology; Forests and forestry -- Periodicals; Forests and forestry -- Japan -- Periodicals; 634.905
• DOI: 10.1080/13416979.2022.2062815
• Languages: English
• ISSNs: 1341-6979

Abstract

ABSTRACT: Extraction of water from soil and plants for isotope analysis is a key step in hydrological and ecological studies. Current water extraction systems are either inefficient or complex and expensive, and depletion of heavy isotopes such as 2 H (deuterium) in extracted water relative to spiked water is commonly observed. We constructed a simple water extraction system using commercially available parts and assessed its sample capacity and performance. We spiked 1% 1 H 2 HO aliquots into soil and plant substrates and analyzed water recovery and isotope exchange of deuterium in spiked water with exchangeable hydrogen in substrates. The water extraction system required 3 days to extract 100% of the spiked water volume when heated at 60°C for plant samples and 100°C for soil samples. Deuterium was depleted in extracted water relative to spiked water due to dilution by substrate-derived exchangeable hydrogen. The exchangeable hydrogen content varied among substrates, causing variation in the degree of deuterium depletion. The simple and low-cost system for water extraction from soil and plant samples decreases barriers to isotope analysis. Estimating substrate-derived exchangeable hydrogen is useful for accounting for deuterium depletion during water extraction.