High-performance blood plasma separation based on a Janus membrane technique and RBC agglutination reaction. Issue 22 (24th October 2022)

• Record Type: Journal Article
• Authors: Xu, Bing; Zhang, Juan; Pan, Deng; Ni, Jincheng; Yin, Kun; Zhang, Qilun; Ding, Yinlong; Li, Ang; Wu, Dong; Shen, Zuojun
• Is Part Of: Lab on a chip. Volume 22:Issue 22(2022)
• Page Start: 4382
• Page End: 4392
• Publication Date: 2022-10-24
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/d2lc00508e
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : A high-performance plasma separation technique is reported to separate plasma from whole blood (hematocrit level: 15–85%) with an ultra-high plasma yield (∼80%) and purity (99.99%) in ∼80 s. Abstract : Separation of plasma which is full of various biomarkers is critical for clinical diagnosis. However, the point-of-care plasma separation often relies on microfluidic filtration membranes which are usually limited in purity, yield, hemolysis, extraction speed, hematocrit level, and protein recovery. Here, we have developed a high-performance plasma membrane separation technique based on a Janus membrane and red blood cell (RBC) agglutination reaction. The RBC agglutination reaction can form larger RBC aggregates to separate plasma from blood cells. Then, the Janus membrane, serving as a multipore microfilter to block large RBC aggregates, allows the plasma to flow from the hydrophobic side to its hydrophilic side spontaneously. As a result, the separation technique can extract highly-purified plasma (99.99%) from whole blood with an ultra-high plasma yield (∼80%) in ∼80 s. Additionally, the separation technique is independent of the hematocrit level and can avoid hemolysis.