Portibacter marinus sp. nov., isolated from the sediment on the surface of plastics and proposal of a novel genus Neolewinella gen. nov. based on the genome-based phylogeny of the family Lewinellaceae. Issue 11 (2nd November 2022)

• Record Type: Journal Article
• Authors: Huang, Zhaobin; Hong, Qianling; Lai, Qiliang; Zhang, Qiufang
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 11(2022)
• Publication Date: 2022-11-02
• Subjects: Lewinella -- Neolewinella -- phylogeny -- polyphasic taxonomy -- Portibacter; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005561
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, rod-shaped and orange-pigmented bacterial strain designated 10MBP4-2-1 T was isolated from the sediment on the surface of a plastic straw collected from oyster-farming areas in Quanzhou Bay, PR China. Catalase activity and oxidase activity were positive. Flexirubin-type pigment was absent. The 16S rRNA gene of strain 10MBP4-2-1 T showed highest sequence similarity to Portibacter lacus YM8-076 T of 98.3 %. Phylogenetic analysis based on 16S rRNA gene sequences and 120 conserved concatenated proteins indicated that strain 10MBP4-2-1 T was affiliated to the genus Portibacter and formed a monophyletic clade with P. lacus YM8-076 T . The digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values between strain 10MBP4-2-1 T and P. lacus YM8-076 T were estimated to be 17.7, 70.4 and 70.3 %, respectively. The respiratory quinone was menaquinone 7. The major fatty acid composition was iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1 ω 7 c and/or C16 : 1 ω 6 c ). The draft genome size was 5 191 941 bp with DNA G+C content of 39.2 %. Based on phylogenetic analyses and whole genomic comparisons, strain 10MBP4-2-1 T represents a novel species, for which the name Portibacter marinus sp. nov. is proposed. The type strain is 10MBP4-2-1 T (=MCCC 1K07073 T =KCTC 92101 T ). Additionally, phylogeny and whole genomic comparison of the family Lewinellaceae placed Lewinella cohaerens and the remaining Lewinella (currently comprising 11 species) in two clearly distinguishable clades recognized at the genus level. Thus, a novel genus named Neolewinella gen. nov. is proposed to accommodate the 11 species. Our study provides a taxonomic framework for the family Lewinellaceae based on genomic data.