An improved carbapenem inactivation method, CIMTrisII, for carbapenemase production by Gram‐negative pathogens. Issue 2 (February 2019)

• Record Type: Journal Article
• Authors: Uechi, Kohei; Tada, Tatsuya; Kuwahara‐Arai, Kyoko; Sekiguchi, Jun‐ichiro; Yanagisawa, Izumi; Tome, Takaaki; Nakasone, Isamu; Maeda, Shiro; Mya, San; Zan, Khin Nyein; Tin, Htay Htay; Kirikae, Teruo; Fujita, Jiro
• Is Part Of: Journal of medical microbiology. Volume 68:Issue 2(2019)
• Publication Date: 2019-02
• Subjects: Carbapenem resistance -- Carbapenemase -- Carbapenem inactivation method -- Enterobacteriaceae -- Acinetobacter species -- Pseudomonas species; Medical microbiology -- Periodicals; 616.9041
• DOI: 10.1099/jmm.0.000888
• Languages: English
• ISSNs: 0022-2615

Abstract

Abstract : N/A: The modified carbapenem inactivation method (mCIM) is a simple phenotypic screening method for detecting carbapenemase production by Enterobacteriaceae and Pseudomonas aeruginosa . We recently developed another modified carbapenem inactivation method (CIMTris), in which carbapenemase is extracted from bacteria with Tris‐HCl buffer, to detect carbapenemase production by Acinetobacter and Pseudomonas species. This study describes an improved carbapenem inactivation method, CIMTrisII, for detecting carbapenemase production by Gram‐negative pathogens, including Enterobacteriaceae, Acinetobacter and Pseudomonas species. CIMTrisII was different from CIMTris in the concentration of Meropenem disks (5‐μg MEM disks vs. 10‐μg MEM disks), the inoculum volume of the bacteria (a 5‐μl loopful vs. a 10 μl loopful) and the incubation time (1 vs. 2 h). CIMTrisII showed an overall sensitivity of 99.3 % and an overall specificity of 95.0 % for tested isolates. In comparison, CIMTris showed a sensitivity of 96.1 % and a specificity of 96.3 %, and mCIM showed a sensitivity of 67.1 % and a specificity of 100 %. CIMTrisII is thus deemed useful for detecting carbapenemase production by Gram‐negative pathogens.