Preparation and hemostatic mechanism of bioactive glass-based membrane-like structure camouflage composite particles. (November 2022)

• Record Type: Journal Article
• Authors: Zheng, Caiyun; Liu, Jinxi; Bai, Que; Quan, Yanxiao; Li, Zihao; Chen, Wenting; Gao, Qian; Zhang, Yanni; Lu, Tingli
• Is Part Of: Materials & design. Volume 223(2022)
• Publication Date: 2022-11
• Subjects: Bioactive glass -- Macroporous and mesoporous -- Membrane-like structure camouflaged -- Composite particles -- Hemostatic mechanism; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2022.111116
• Languages: English
• ISSNs: 0264-1275

Abstract

Graphical abstract: Bioactive glass-based membrane-like structure camouflage composite particles (MBG@BSA/CS) were prepared by Layer-by-layer (LBL) method to coat bovine serum albumin (BSA) and chitosan (CS) on the surface of the synthesized macroporous and mesoporous bioactive glass (MBG). The MBG-based membrane-like structure camouflaged particles activated both endogenous and exogenous physiological coagulation pathways and could immediately release Ca 2+ to participate in the coagulation pathway. MBG@BSA/CS could form a fibrin network by aggregating erythrocytes and activating platelets, thus rapidly aggregating coagulation factors, which had a significant hemostatic effect on surface irregular bleeding (tail-amputation model) and internal irregular and deep bleeding (liver injury model) in SD rats. Compared with MBG, MBG@BSA/CS effectively improved hemostatic efficiency in animal injury model experiments. Highlights: The bioactive glass-based membrane-like structure camouflage composite particles were prepared by Layer-by-layer method. The membrane-like structure accelerated platelet activation and erythrocyte aggregation. The membrane-like structure camouflage composite particles triggered both endogenous and exogenous coagulation pathway. The composite particles could be used for the treatment of bleeding in the irregular or deep wounds. Abstract: Powder hemostatic particles have no limitation on wound type and a wide application prospect in the treatment of wound hemostasis. In this study, bioactive glass-based membrane-like structure camouflage composite particles (MBG@BSA/CS) were prepared by Layer-by-layer (LBL) method to coat bovine serum albumin (BSA) and chitosan (CS) on the surface of the synthesized macroporous and mesoporous bioactive glass (MBG). The results showed that MBG@BSA/CS particles significantly improved the coagulation effect of MBG and could activated both endogenous and exogenous physiological coagulation pathways. MBG@BSA/CS had the excellent water absorption quality and could immediately release Ca 2+ to participate in the coagulation pathway. The main hemostatic mechanism of MBG@BSA/CS was that the membrane-like of BSA/CS could form a fibrin network by aggregating erythrocytes and activating platelets, thus rapidly aggregating coagulation factors. In addition, MBG@BSA/CS particles had a significant hemostatic effect on surface bleeding (tail-amputation model) and internal bleeding (liver injury model) in SD rats, which could shorten the bleeding time and reduce the amount of blood loss effectively. This research work stated that the MBG-based membrane-like structure camouflage composite particles might be with the potential applications as a hemostasis biomaterial for the emergency bleeding.