Advances in Lentivirus Purification. Issue 1 (8th December 2020)

• Record Type: Journal Article
• Authors: Moreira, Ana Sofia; Cavaco, David Guia; Faria, Tiago Q.; Alves, Paula M.; Carrondo, Manuel J. T.; Peixoto, Cristina
• Is Part Of: Biotechnology journal. Volume 16:Issue 1(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-12-08
• Subjects: bioseparation -- chromatography -- downstream processing -- lentiviral vectors; Biotechnology -- Periodicals; 660.605
• DOI: 10.1002/biot.202000019
• Languages: English
• ISSNs: 1860-6768

Abstract

Abstract: Lentiviral vectors (LVs) have been increasingly used as a tool for gene and cell therapies since they can stably integrate the genome in dividing and nondividing cells. LV production and purification processes have evolved substantially over the last decades. However, the increasing demands for higher quantities with more restrictive purity requirements are stimulating the development of novel materials and strategies to supply the market with LV in a cost‐effective manner. A detailed review of each downstream process unit operation is performed, limitations, strengths, and potential outcomes being covered. Currently, the majority of large‐scale LV manufacturing processes are still based on adherent cell culture, although it is known that the industry is migrating fast to suspension cultures. Regarding the purification strategy, it consists of batch chromatography and membrane technology. Nevertheless, new solutions are being created to improve the current production schemes and expand its clinical use. Abstract : Lentiviral vectors (LVs) are increasingly being used as a gene delivery tool for gene and cell therapies. In this study, the authors review the current methods used in LV downstream processing and some of the new emerging technologies. Future trends are outlined considering the manufacturing needs of growing market.