Integrin‐Targeted, Short Interfering RNA Nanocomplexes for Neuroblastoma Tumor‐Specific Delivery Achieve MYCN Silencing with Improved Survival. (30th June 2021)

• Record Type: Journal Article
• Authors: Tagalakis, Aristides D.; Jayarajan, Vignesh; Maeshima, Ruhina; Ho, Kin H.; Syed, Farhatullah; Wu, Lin‐Ping; Aldossary, Ahmad M.; Munye, Mustafa M.; Mistry, Talisa; Ogunbiyi, Olumide Kayode; Sala, Arturo; Standing, Joseph F.; Moghimi, Seyed M.; Stoker, Andrew W.; Hart, Stephen L.
• Is Part Of: Advanced functional materials. Volume 31:Number 37(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-06-30
• Subjects: MYCN -- neuroblastomas -- siRNA -- tumor‐specific delivery -- tumors; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.202104843
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: The authors aim to develop siRNA therapeutics for cancer that can be administered systemically to target tumors and retard their growth. The efficacy of systemic delivery of siRNA to tumors with nanoparticles based on lipids or polymers is often compromised by their rapid clearance from the circulation by the liver. Here, multifunctional cationic and anionic siRNA nanoparticle formulations are described, termed receptor‐targeted nanocomplexes (RTNs), that comprise peptides for siRNA packaging into nanoparticles and receptor‐mediated cell uptake, together with lipids that confer nanoparticles with stealth properties to enhance stability in the circulation, and fusogenic properties to enhance endosomal release within the cell. Intravenous administration of RTNs in mice leads to predominant accumulation in xenograft tumors, with very little detected in the liver, lung, or spleen. Although non‐targeted RTNs also enter the tumor, cell uptake appears to be RGD peptide‐dependent indicating integrin‐mediated uptake. RTNs with siRNA against MYCN (a member of the Myc family of transcription factors) in mice with MYCN ‐amplified neuroblastoma tumors show significant retardation of xenograft tumor growth and enhanced survival. This study shows that RTN formulations can achieve specific tumor‐targeting, with minimal clearance by the liver and so enable delivery of tumor‐targeted siRNA therapeutics. Abstract : This paper describes a new lipid and peptide nanoparticle formulation with potential as a treatment for neuroblastoma that targets tumors specifically by intravenous administration. The nanoparticle delivers a siRNA molecule that silences the proto‐oncogene MYCN as a neuroblastoma tumor‐specific target. The nanoparticle accumulates specifically in tumors in mice and the treatment leads to retarded tumor growth in most cases.