Neutrophils induce paracrine telomere dysfunction and senescence in ROS‐dependent manner. (25th March 2021)

• Record Type: Journal Article
• Authors: Lagnado, Anthony; Leslie, Jack; Ruchaud‐Sparagano, Marie‐Helene; Victorelli, Stella; Hirsova, Petra; Ogrodnik, Mikolaj; Collins, Amy L; Vizioli, Maria Grazia; Habiballa, Leena; Saretzki, Gabriele; Evans, Shane A; Salmonowicz, Hanna; Hruby, Adam; Geh, Daniel; Pavelko, Kevin D; Dolan, David; Reeves, Helen L; Grellscheid, Sushma; Wilson, Colin H; Pandanaboyana, Sanjay; Doolittle, Madison; von Zglinicki, Thomas; Oakley, Fiona; Gallage, Suchira; Wilson, Caroline L; Birch, Jodie; Carroll, Bernadette; Chapman, James; Heikenwalder, Mathias; Neretti, Nicola; Khosla, Sundeep; Masuda, Claudio Akio; Tchkonia, Tamar; Kirkland, James L; Jurk, Diana; Mann, Derek A; Passos, João F;
• Is Part Of: EMBO journal. Volume 40:Number 9(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-03-25
• Subjects: aging -- neutrophils -- senescence -- telomeres; Molecular biology -- Periodicals; 572.805
• DOI: 10.15252/embj.2020106048
• Languages: English
• ISSNs: 0261-4189

Abstract

Abstract: Cellular senescence is characterized by an irreversible cell cycle arrest as well as a pro‐inflammatory phenotype, thought to contribute to aging and age‐related diseases. Neutrophils have essential roles in inflammatory responses; however, in certain contexts their abundance is associated with a number of age‐related diseases, including liver disease. The relationship between neutrophils and cellular senescence is not well understood. Here, we show that telomeres in non‐immune cells are highly susceptible to oxidative damage caused by neighboring neutrophils. Neutrophils cause telomere dysfunction both in vitro and ex vivo in a ROS‐dependent manner. In a mouse model of acute liver injury, depletion of neutrophils reduces telomere dysfunction and senescence. Finally, we show that senescent cells mediate the recruitment of neutrophils to the aged liver and propose that this may be a mechanism by which senescence spreads to surrounding cells. Our results suggest that interventions that counteract neutrophil‐induced senescence may be beneficial during aging and age‐related disease. SYNOPSIS: Whether infiltrating immune cells impact on tissue viability during inflammatory response is poorly characterized. Here, activated neutrophils are shown to induce ROS‐dependent acute senescence in adjacent naïve cells, raising the possibility of intercellular senescence crosstalk during ageing and age‐related pathologies. Neutrophils induce paracrine senescence in neighbouring cells via ROS‐dependent telomere dysfunction. Neutrophil clearance prevents senescence and telomere dysfunction in a model of acute liver injury. Senescent cells recruit neutrophils via the senescence‐associated secretory phenotype (SASP) in the aged liver. Abstract : Oxidative damage caused by inflammatory neutrophils contributes to neighbouring cell senescence and liver injury.