Trypsin Inhibitor Isolated From Glycine max (Soya Bean) Extraction, Purification, and Characterization. Issue 4 (4th October 2022)

• Record Type: Journal Article
• Authors: Khan, Sabir; Arshad, Shafia; Arif, Amina; Tanveer, Rida; Amin, Zeemal Seemab; Abbas, Saba; Maqsood, Amna; Raza, Muhammad; Munir, Arooj; Latif, Amara; Habiba, Maryam; Afzal, Muhammad
• Is Part Of: Dose-response. Volume 20:Issue 4(2022)
• Publication Date: 2022-10-04
• Subjects: Bowman-Birk trypsin inhibitor -- Kunitz trypsin inhibitor -- soybean trypsin inhibitor and antioxidant activity; Dose-response relationship (Biochemistry) -- Periodicals; Drugs -- Dose-response relationship -- Periodicals; Drugs -- Physiological effect -- Periodicals; Hormesis -- Periodicals; Dose-Response Relationship, Drug -- Periodicals; Dose-response relationship (Biochemistry); Drugs -- Dose-response relationship; Drugs -- Physiological effect; Periodicals; 571.634
• DOI: 10.1177/15593258221131462
• Languages: English
• ISSNs: 1559-3258

Abstract

The current study aims to isolate, purify, and characterize the trypsin inhibitor protein from seeds of soya beans, scientifically known as Glycine max . Its seeds were ground, and the powder was soaked several times using n-hexane. It was added to phosphate buffer saline (PBS) followed by filtration and centrifugation of the PBS dissolved extract. The supernatant was subjected to ammonium sulfate precipitation and about six fractions, 30% to 80% were prepared. The centrifuged pellets obtained from each fraction were dialyzed and run on SDS-PAGE. The trypsin inhibitor protein was precipitated and characterized in 30% pellet and molecular weight was 21.5 kDa compared to protein ladder (ThermoFisher 10-170 kDa). GC-MS analysis revealed the steroid derivatives such as stigmasterol, campesterol, beta-sitosterol, and gamma-tocopherol. Glycine max trypsin inhibitor could be used as a plant-derived drug to overcome the over-activation of trypsin without its real substrate (proteins) becoming activated and start auto digestion leading to pancreatitis. Graphical Abstract