Precision cut tissue slices to investigate the effects of triclosan exposure in Mytilus galloprovincialis. (December 2022)

• Record Type: Journal Article
• Authors: Hosseinzadeh, Mahaboubeh; Gilabert, Alejandra; Porte, Cinta
• Is Part Of: Toxicology in vitro. Volume 85(2023)
• Publication Date: 2022-12
• Subjects: PCTS -- Triclosan -- Carboxylesterases -- Lipid metabolism; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2022.105477
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Precision-cut tissue slices (PCTS) are frequently used in mammalian research, but its application in the area of aquatic toxicology is still humble. This work proposes the use of PCTS to investigate the effects of the antimicrobial triclosan (TCS) in the mussel Mytilus galloprovincialis . PCTS sectioned from the digestive gland (400 μm) were exposed to 10, 100, and 500 nM TCS for 24 h, and the expression of selected genes, together with the biomarkers, carboxylesterases (CbE) and glutathione S-transferases (GST), and the analysis of lipids in PCTS and culture medium, were used to investigate the molecular initiating events of triclosan in the digestive gland of mussels. Significant dysregulation in the expression of phenylalanine-4-hydroxylase (PAH), glutamate dehydrogenase (GDH), fatty acid synthase (FASN), and 7-dehydrocholesterol reductase (DHCR7), involved in energy, phenylalanine and lipid metabolism, were detected. The analysis of lipids evidenced significant changes in cholesteryl esters (CEs) and membrane lipids in the culture medium of exposed PCTS, suggesting dysregulation of energy and lipid metabolism that can affect lipid dynamics in mussels exposed to triclosan. Highlights: Digestive gland PCTS are a suitable ex vivo model for toxicological studies. Different molecular responses can be successfully determined in PCTS. TCS exposure upregulates genes related to energy and nitrogen metabolism. TCS downregulates genes related to cholesterol and fatty acid synthesis.