Bioprospecting of novel peroxidase from Streptomyces coelicolor strain SPR7 for carcinogenic azo dyes decolorization. (January 2023)

• Record Type: Journal Article
• Authors: Preethi, P. Sai; Vickram, Sundaram; Das, Raja; Hariharan, N.M.; Rameshpathy, M.; Subbaiya, R.; Karmegam, N.; Kim, Woong; Govarthanan, M.
• Is Part Of: Chemosphere. Volume 310(2023)
• Publication Date: 2023-01
• Subjects: Azo dyes -- Back propagation algorithm -- Computational docking -- Peroxidase -- Statistical modeling -- Streptomyces coelicolor; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.136836
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Peroxidase (POX) is a heme-containing oxidoreductase, its voluminous immuno-diagnostic and bioremediatory intuitions have incited optimization and large scale-generation from novel microbial repertoires. Azo dyes are the most detrimental classes of synthetic dyes and they are the common ecotoxic industrial pollutants in wastewater. In addition, azo dyes are refractory to degradation owing to their chemical nature, comprising of azoic linkages, amino moieties with recalcitrant traits. Moreover, they are major carcinogenic and mutagenic on humans and animals, whereby emphasizing the need for decolorization. In the present study, a novel POX from Streptomyces coelicolor strain SPR7 was investigated for the deterioration of ecotoxic dyestuffs. The initial medium component screening for POX production was achieved using, One Factor at a Time and Placket-Burman methodologies with starch, casein and temperature as essential parameters. In auxiliary, Response Surface Methodology (RSM) was recruited and followed by model validation using Back propagation algorithm (BPA). RSM-BPA composite approach prophesied that combination of starch, casein, and temperature at optimal values 2.5%, 0.035% and 35 °C respectively, has resulted in 7 folds enhancement of POX outturn (2.52 U/mL) compared to the unoptimized media (0.36 U/mL). The concentrated enzyme decolorized 75.4% and 90% of the two azo dyes with lignin (10 mM), respectively. Hence, this investigation confirms the potentiality of mangrove actinomycete derived POX for elimination of noxious azo dyes to overcome their carcinogenic, mutagenic and teratogenic effects on humans and aquatic organisms. Graphical abstract: Image 1 Highlights: Novel peroxidase (POX) from Streptomyces coelicolor was bioprospected. Response Surface and Artificial intelligence strategies are delineated. Carcinogenic Direct Red 23 and Direct Blue 15 dyes were targeted with POX. Cutting-edge treatment by in silico cum in vitro validations were projected. POX decolorized 75.4% Direct Red 23 and 90% Direct Blue 15 with lignin.