An enriched ammonia-oxidizing microbiota enables high removal efficiency of ammonia in antibiotic production wastewater. (January 2023)

• Record Type: Journal Article
• Authors: Chen, Jiamin; Liang, Jiawei; Li, Chenjing; Dai, Jihua; Mai, Wenning; Wei, Yongjun
• Is Part Of: Chemosphere. Volume 310(2023)
• Publication Date: 2023-01
• Subjects: Partial nitrification -- Ammonia-oxidizing bacteria -- Microbiota -- Abamectin production wastewater -- Lincomycin production wastewater; APW antibiotic production wastewater -- AOB ammonium-oxidizing bacteria -- AOR ammonia oxidation rate -- ARE ammonium nitrogen removal efficiency -- COD chemical oxygen demand -- DO dissolved oxygen -- FA free ammonium -- FNA free nitrous acid -- LM liquid medium -- MLSS mixed liquor suspended solids -- MLVSS mixed liquor volatile suspended solids -- NAR nitrite-nitrogen accumulation rate -- NH4+-N ammonia nitrogen -- NO2−-N nitrite nitrogen -- NO3−-N nitrate nitrogen -- NOB nitrite-oxidizing bacteria -- SAOR specific ammonia oxidation rate -- TN total nitrogen; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.136854
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: High ammonia concentration hinders the efficient treatment of antibiotic production wastewater (APW). Developing effective ammonia oxidation wastewater treatment strategies is an ideal approach for facilitating APW treatment. Compared with traditional nitrification strategies, the partial nitrification process is more eco-friendly, less energy-intensive, and less excess sludge. The primary limiting factor of the partial nitrification process is increasing ammonia-oxidizing bacteria (AOB) while decreasing nitrite-oxidizing bacteria (NOB). In this study, an efficient AOB microbiota (named AF2) was obtained via enrichment of an aerobic activated sludge (AS0) collected from a pharmaceutical wastewater treatment plant. After a 52-day enrichment of AS0 in 250 mL flasks, the microbiota AE1 with 69.18% Nitrosomonas microorganisms was obtained. Subsequent scaled-up cultivation in a 10 L fermenter led to the AF2 microbiota with 59.22% Nitrosomonas . Low concentration of free ammonia (FA, < 42.01 mg L −1 ) had a negligible effect on the activity of AF2, and the nitrite-nitrogen accumulation rate (NAR) of AF2 was 98% when FA concentration was 42.01 mg L −1 . The specific ammonia oxidation rates (SAORs) at 30 °C and 15 °C were 3.64 kg NH4 + -N·kg MLVSS −1 ·d −1 and 1.43 kg NH4 + -N·kg MLVSS −1 ·d −1 (MLVSS: mixed liquor volatile suspended solids). The SAOR was 0.52 kg NH4 + -N·kg MLVSS −1 ·d −1 when the NaCl concentration was increased from 0 to 20 g L −1, showing that AF2 functioning was stable in a high-level salt environment. The ammonia oxidation performance of AF2 was verified by treating abamectin and lincomycin production wastewater. The NARs of AF2 used for abamectin and lincomycin production wastewater treatment were >90% and the SAORs were 2.39 kg NH4 + -N·kg MLVSS −1 ·d −1 and 0.54 kg NH4 + -N·kg MLVSS −1 ·d −1, respectively, which was higher than the traditional biological denitrification process. In summary, AF2 was effective for APW treatment via enhanced ammonia removal efficiency, demonstrating great potential for future industrial wastewater treatment. Graphical abstract: Image 1 Highlights: The enrichment strategy for simplified efficient AOB microbiota was introduced. One microbiota dominated with Nitrosomonas was successfully built. The microbiota was stable under several different environmental parameters. The microbiota can efficiently remove ammonia pollutants in antibiotic production wastewater.