Fabrication of microgroove poly(lactic-co-glycolic acid) nerve guide conduit using dry-jet wet spinning for rat laryngeal recurrent nerve regeneration. (November 2022)

• Record Type: Journal Article
• Authors: Yang, Xiaohui; Liu, Xixia; Xu, Fangshen; Ji, Shunxian; Sun, Yuchao; Song, Zhaojun; Song, Jie; Wu, Yulian; Yin, Jun
• Is Part Of: Materials & design. Volume 223(2022)
• Publication Date: 2022-11
• Subjects: Nerve guide conduit -- Recurrent laryngeal nerve regeneration -- Microgroove structure -- Poly(lactic-co-glycolic acid) -- Dry-jet wet spinning; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2022.111151
• Languages: English
• ISSNs: 0264-1275

Abstract

Graphical abstract: Highlights: PLGA nerve guide conduits with smooth and micro-grooved inner surfaces were fabricated by dry-jet wet spinning. Rat recurrent laryngeal nerve injury model was established to evaluate the repair efficiency of different nerve guide conduits. The micro-grooved nerve guide conduits could significantly improve the recurrent laryngeal nerve regeneration. Abstract: Recurrent laryngeal nerve (RLN) injury is a serious complication of thyroidectomy. Here, the degradable poly(lactic- co -glycolic acid) (PLGA) nerve guide conduits (NGCs) with microgroove or smooth inner surface were fabricated using a phase inversion-based dry-jet wet spinning method for RLN regeneration. The morphology and mechanical properties of NGCs were observed by scanning electron microscope (SEM) and a universal testing machine, showing that the porous NGC has good mechanical properties and is suitable for implantation. Further, in vitro PC12 cells cultured on the NGCs showed that microgroove conduit (GNGC) exhibited better cell adhesion, proliferation, and cell orientation. Both smooth and microgroove conduit (SNGC and GNGC) were transplanted into Sprague-Dawley (SD) rat models for assessing the repair effect on the 5-mm RLN gap. The results showed that GNGC was superior to SNGC in nerve regeneration and had similar effects to the autograft group in reducing muscle atrophy and restoring target organ function. Therefore, the degradable NGCs combined with groove structure could guide the regeneration of injured RLN, promote the partial functional recovery of target organs and prevent secondary surgical injury. This study contributes to the design and fabrication of nerve guide conduits and the application of tissue engineering in the field of RLN injury repair.