Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism. Issue 12 (11th January 2022)

• Record Type: Journal Article
• Authors: Belda, Eugeni; Voland, Lise; Tremaroli, Valentina; Falony, Gwen; Adriouch, Solia; Assmann, Karen E; Prifti, Edi; Aron-Wisnewsky, Judith; Debédat, Jean; Le Roy, Tiphaine; Nielsen, Trine; Amouyal, Chloé; André, Sébastien; Andreelli, Fabrizio; Blüher, Matthias; Chakaroun, Rima; Chilloux, Julien; Coelho, Luis Pedro; Dao, Maria Carlota; Das, Promi; Fellahi, Soraya; Forslund, Sofia; Galleron, Nathalie; Hansen, Tue H; Holmes, Bridget; Ji, Boyang; Krogh Pedersen, Helle; Le, Phuong; Le Chatelier, Emmanuelle; Lewinter, Christian; Mannerås-Holm, Louise; Marquet, Florian; Myridakis, Antonis; Pelloux, Veronique; Pons, Nicolas; Quinquis, Benoit; Rouault, Christine; Roume, Hugo; Salem, Joe-Elie; Sokolovska, Nataliya; Søndertoft, Nadja B; Touch, Sothea; Vieira-Silva, Sara; Galan, Pilar; Holst, Jens; Gøtze, Jens Peter; Køber, Lars; Vestergaard, Henrik; Hansen, Torben; Hercberg, Serge; Oppert, Jean-Michel; Nielsen, Jens; Letunic, Ivica; Dumas, Marc-Emmanuel; Stumvoll, Michael; Pedersen, Oluf Borbye; Bork, Peer; Ehrlich, Stanislav Dusko; Zucker, Jean-Daniel; Bäckhed, Fredrik; Raes, Jeroen; Clément, Karine;
• Other Names: author non-byline.; Alili Rohia author non-byline.; Alves Renato author non-byline.; Astrid Ehm author non-byline.; Galijatovic Andersson author non-byline.; Barthelemy Olivier author non-byline.; Bastard Jean-Philippe author non-byline.; Batisse Jean-Paul author non-byline.; Berland Magalie author non-byline.; Bittar Randa author non-byline.; Blottière Hervé author non-byline.; Bosquet Frederic author non-byline.; Boubrit Rachid author non-byline.; Bourron Olivier author non-byline.; Camus Mickael author non-byline.; Cassuto Dominique author non-byline.; Ciangura Cecile author non-byline.; Collet Jean-Philippe author non-byline.; Dietrich Arne author non-byline.; Djebbar Morad author non-byline.; Doré Angélique author non-byline.; Engelbrechtsen Line author non-byline.; Fezeu Leopold author non-byline.; Fromentin Sebastien author non-byline.; Giral Philippe author non-byline.; Graine Marianne author non-byline.; Grünemann Caroline author non-byline.; Hartemann Agnes author non-byline.; Hartmann Bolette author non-byline.; Helft Gerard author non-byline.; Hornbak Malene author non-byline.; Hoyles Lesley author non-byline.; Hulot Jean-Sebastien author non-byline.; Isnard Richard author non-byline.; Jaqueminet Sophie author non-byline.; Jørgensen Niklas Rye author non-byline.; Julienne Hanna author non-byline.; Justesen Johanne author non-byline.; Kammer Judith author non-byline.; Karup Nikolaj author non-byline.; Kerneis Mathieu author non-byline.; Khemis Jean author non-byline.; Køber Lars author non-byline.; Kozlowski Ruby author non-byline.; Kuhn Michael author non-byline.; Lampure Aurelie author non-byline.; Lejard Véronique author non-byline.; Letunic Ivica author non-byline.; Levenez Florence author non-byline.; Marko Lajos author non-byline.; Lucas-Martini Lea author non-byline.; Martinez-Gili Laura author non-byline.; Massey Robin author non-byline.; Maziers Nicolas author non-byline.; Medina-Stamminger Jonathan author non-byline.; Moitinho-Silva Lucas author non-byline.; Montalescot Gilles author non-byline.; Moutel Sandrine author non-byline.; Neves Ana Luisa author non-byline.; Olanipekun Michael author non-byline.; Pavin Laetitia Pasero Le author non-byline.; Coelho Luis Pedro author non-byline.; Poitou Christine author non-byline.; Pousset Francoise author non-byline.; Pouzoulet Laurence author non-byline.; Rodriguez-Martinez Andrea author non-byline.; Schmidt Sebastien author non-byline.; Schütz Tatjana author non-byline.; Silva Lucas author non-byline.; Silvain Johanne author non-byline.; Svendstrup Mathilde author non-byline.; Swartz Timothy D author non-byline.; Vanduyvenboden Thierry author non-byline.; Vatier Camille author non-byline.; Verger Eric O author non-byline.; Walther Stefanie author non-byline.;
• Is Part Of: Gut. Volume 71:Issue 12(2022)
• Page Start: 2463
• Page End: 2480
• Publication Date: 2022-01-11
• Subjects: obesity -- micronutrients -- nutrition -- intestinal bacteria -- diabetes mellitus; Gastroenterology -- Periodicals; 616.33
• DOI: 10.1136/gutjnl-2021-325753
• Languages: English
• ISSNs: 0017-5749

Abstract

Abstract : Objectives: Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear. We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort. We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation. Design: We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice. Results: Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes. We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue. In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels. Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice. Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration. Conclusion: Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity. Trial registration number: NCT02059538 .