Wound swabs versus biopsies to detect methicillin resistant Staphylococcus aureus in experimental equine wounds. Issue 8 (14th September 2022)

• Record Type: Journal Article
• Authors: Brock, Abbi K.; Chamoun‐Emanuelli, Ana M.; Howard, Emily A.; Huntzinger, Katie D.; Lawhon, Sara D.; Bryan, Laura K.; Cosgriff‐Hernandez, Elizabeth M.; Cohen, Noah D.; Whitfield‐Cargile, Canaan M.
• Is Part Of: Veterinary surgery. Volume 51:Issue 8(2022)
• Page Start: 1196
• Page End: 1205
• Publication Date: 2022-09-14
• Subjects: Veterinary surgery -- Periodicals; Veterinary Medicine -- Periodicals; Surgery -- Periodicals; Societies, Medical -- Periodicals; 636.0897
• DOI: 10.1111/vsu.13872
• Languages: English
• ISSNs: 0161-3499

Abstract

Abstract: Objective: To compare: (1) the load and diversity of cultivatable bacterial species isolated from tissue biopsies with cultures from surface swabs, and (2) the ability of each technique to detect methicillin‐resistant Staphylococcus aureus (MRSA) in a model of MRSA‐infected equine wounds. Study design: Experimental in vivo study. Animals: Three light‐breed adult horses. Methods: Four 2.5 × 2.5 cm full‐thickness skin wounds were created on the dorsolateral aspect of each forelimb. Five days later, each wound was inoculated with a pure culture of MRSA (ATCC 43300). One hundred microlitres of 0, 5 × 10 8, 5 × 10 9 or 5 × 10 10 colony forming units (CFU)/ml was used to inoculate each wound. Surface swabs (Levine technique) and tissue biopsy samples (3 mm punch biopsy) were obtained at 2, 7, 14, and 21 days after inoculation. Quantitative aerobic culture was performed using routine clinical techniques. Results: A similar bacterial profile was identified from the culture of each wound‐sampling technique and there was moderate correlation ( R = 0.49, P < .001) between the bacterial bioburdens. Agreement was fair ( κ = 0.31; 95% CI, 0.129‐0.505) between the sampling techniques in identification of MRSA. Methicillin‐resistant Staphylococcus aureus was isolated more frequently ( P = .016) from cultures of tissue biopsies (79%; 76/96) than from surface swabs (62%; 60/96). Conclusion: Bacterial load and diversity did not differ between sampling techniques but MRSA was detected more often from the cultures of tissue biopsies. Clinical significance: Tissue biopsy should be preferred to culture swab in wounds where MRSA is suspected.