ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation. Issue 11 (23rd September 2022)

• Record Type: Journal Article
• Authors: Hiraoka, Haruka; Wang, Jiewen; Nakano, Tadashi; Hirano, Yasuhiro; Yamazaki, Shinichi; Hiraoka, Yasushi; Haraguchi, Tokuko
• Other Names: Rizo Josep guestEditor.
• Is Part Of: FEBS open bio. Volume 12:Issue 11(2022)
• Page Start: 2042
• Page End: 2056
• Publication Date: 2022-09-23
• Subjects: ATP -- cell movement -- Dictyostelium -- differentiation -- live‐cell imaging -- simulation; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.13480
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Cell migration plays an important role in multicellular organism development. The cellular slime mold Dictyostelium discoideum is a useful model organism for the study of cell migration during development. Although cellular ATP levels are known to determine cell fate during development, the underlying mechanism remains unclear. Here, we report that ATP‐rich cells efficiently move to the central tip region of the mound against rotational movement during the mound phase. A simulation analysis based on an agent‐based model reproduces the movement of ATP‐rich cells observed in the experiments. These findings indicate that ATP‐rich cells have the ability to move against the bulk flow of cells, suggesting a mechanism by which high ATP levels determine the cell fate of differentiation. Abstract : Cell movement was analyzed during the development of Dictyostelium discoideum . During the mound phase, ATP‐rich cells efficiently move toward the center in a spiral manner against the rotational motion of ATP‐poor cells. ATP‐rich cells are located around the center, and eventually differentiate into stalk cells, suggesting that the centripetal movement of ATP‐rich cells determines the cell fate of differentiation.