MYPT1 inhibits the metastasis of renal clear cell carcinoma via the MAPK8/N‐cadherin pathway. Issue 11 (27th September 2022)

• Record Type: Journal Article
• Authors: Xie, Qingling; Liu, Ren; Zou, Zhihao; Feng, Yuanfa; Huang, Yiqiao; Xu, Guibin; Sun, Wei; Liang, Yuxiang; Zhong, Weide
• Other Names: Rizo Josep guestEditor.
• Is Part Of: FEBS open bio. Volume 12:Issue 11(2022)
• Page Start: 2083
• Page End: 2095
• Publication Date: 2022-09-27
• Subjects: cadherin -- MAPK -- metastasis -- MYPT1 -- renal clear cell carcinoma; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.13487
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Myosin phosphatase target subunit 1 (MYPT1) is a subunit of myosin phosphatase that is capable of regulating smooth muscle contraction. MYPT1 has been reported to be involved in a wide variety of tumours, but its expression and biological functions in renal clear cell carcinoma (ccRCC) remain obscure. Herein, we analysed the relationship between patient clinicopathological characteristics and MYPT1 expression levels in ccRCC patients using a tissue microarray (TMA) and data retrieved from the TCGA‐KIRC dataset. MYPT1 was overexpressed or depleted using siRNA in ccRCC cells to assess the effects on migration and invasion in vitro and in vivo . Additionally, RNA‐sequencing and bioinformatics analysis were performed to investigate the precise mechanism. MYPT1 expression in ccRCC tissues was observed to be lower than that in nonmalignant tissues ( P < 0.05). In addition, MYPT1 downregulation was closely linked to advanced pathological stage ( P < 0.05), and poor OS (overall survival; P < 0.05). Functionally, increased expression of MYPT1 suppressed ccRCC migration and invasion in vitro, and inhibited tumour metastasis in vivo . In addition, MYPT1 overexpression exerted its suppressive effects via the MAPK8/N‐cadherin pathway in ccRCC. Abstract : In this study, a MYPT1‐MAPK8/N‐cadherin axis in tumour cells was revealed to inhibit the metastasis of renal clear cell carcinoma. MYPT1 expression was downregulated in tumour tissues and linked to aggressive disease progression and a poor prognosis in ccRCC patients.