A comparison of spectral unmixing to conventional compensation for the calculation of fluorochrome abundances from flow cytometric data. Issue 11 (15th July 2022)

• Record Type: Journal Article
• Authors: Novo, David
• Other Names: Lannigan Joanne guestEditor.
• Is Part Of: Cytometry. Volume 101:Issue 11(2022)
• Page Start: 885
• Page End: 891
• Publication Date: 2022-07-15
• Subjects: compensation -- regression -- spectral flow cytometry -- unmixing; Flow cytometry -- Periodicals; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnostic imaging -- Periodicals; 571.605
• DOI: 10.1002/cyto.a.24669
• Languages: English
• ISSNs: 1552-4922

Abstract

Abstract: Traditionally, flow cytometers acquired data using the same number of detectors as fluorochromes being measured in the experiment. More recently, spectral flow cytometers utilize a larger number of detectors than fluorochromes. This seemingly small difference opens the door to a wide variety of mathematical tools for the calculation of the true fluorochrome abundances from the raw detector values as compared with traditional compensation. This review will provide a brief overview of the mathematics and theory underlying traditional compensation and unmixing focusing on the differences between them and the additional information provided by unmixing approaches.