Impact of different oral treatments on the composition of the supragingival plaque microbiome. Issue 1 (31st December 2022)

• Record Type: Journal Article
• Authors: Rabe, Alexander; Gesell Salazar, Manuela; Michalik, Stephan; Kocher, Thomas; Below, Harald; Völker, Uwe; Welk, Alexander
• Is Part Of: Journal of oral microbiology. Volume 14:Issue 1(2022)
• Publication Date: 2022-12-31
• Subjects: Teeth -- dental plaque -- metaproteomics -- healthy human oral microbiome -- lactoperoxidase -- essential oil -- nLC-MS/MS; Mouth -- Microbiology -- Periodicals; Medical microbiology -- Periodicals; Mouth Diseases -- microbiology; Mouth -- microbiology; Medical microbiology; Mouth -- Microbiology; Oral Pathology; Microbiology; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; Electronic journals; 616.31
• DOI: 10.1080/20002297.2022.2138251
• Languages: English
• ISSNs: 2000-2297

Abstract

ABSTRACT: Background: Dental plaque consists of a diverse microbial community embedded in a complex structure of exopolysaccharides. Dental biofilms form a natural barrier against pathogens but lead to oral diseases in a dysbiotic state. Objective: Using a metaproteome approach combined with a standard plaque-regrowth study, this pilot study examined the impact of different concentrations of lactoperoxidase (LPO) on early plaque formation, and active biological processes. Design: Sixteen orally healthy subjects received four local treatments as a randomized single-blind study based on a cross-over design. Two lozenges containing components of the LPO-system in different concentrations were compared to a placebo and Listerine®. The newly formed dental plaque was analyzed by mass spectrometry (nLC-MS/MS). Results: On average 1, 916 metaproteins per sample were identified, which could be assigned to 116 genera and 1, 316 protein functions. Listerine® reduced the number of metaproteins and their relative abundance, confirming the plaque inhibiting effect. The LPO-lozenges triggered mainly higher metaprotein abundances of early and secondary colonizers as well as bacteria associated with dental health but also periodontitis. Functional information indicated plaque biofilm growth. Conclusion: In conclusion, the mechanisms on plaque biofilm formation of Listerine® and the LPO-system containing lozenges are different. In contrast to Listerine®, the lozenges led to a higher bacterial diversity.