Genetic architecture underlying HPPD‐inhibitor resistance in a Nebraska Amaranthus tuberculatus population. Issue 11 (10th August 2021)

• Record Type: Journal Article
• Authors: Murphy, Brent P; Beffa, Roland; Tranel, Patrick J
• Is Part Of: Pest management science. Volume 77:Issue 11(2021)
• Page Start: 4884
• Page End: 4891
• Publication Date: 2021-08-10
• Subjects: Amaranthus tuberculatus -- waterhemp -- quantitative trait loci -- herbicide resistance -- HPPD‐inhibitor resistance -- genetic mapping; Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.6560
• Languages: English
• ISSNs: 1526-498X

Abstract

Abstract: BACKGROUND: Amaranthus tuberculatus is a problematic weed species in Midwest USA agricultural systems. Inhibitors of 4‐hydroxyphenylpyruvate dioxygenase (HPPD) are an important chemistry for weed management in numerous cropping systems. Here, we characterize the genetic architecture underlying the HPPD‐inhibitor resistance trait in an A. tuberculatus population (NEB). RESULTS: Dose–response studies of an F1 generation identified HPPD‐inhibitor resistance as a dominant trait with a resistance factor of 15.0–21.1 based on dose required for 50% growth reduction. Segregation analysis in a pseudo‐F2 generation determined the trait is moderately heritable ( H 2 = 0.556) and complex. Bulk segregant analysis and validation with molecular markers identified two quantitative trait loci (QTL), one on each of Scaffold 4 and 12. CONCLUSIONS: Resistance to HPPD inhibitors is a complex, largely dominant trait within the NEB population. Two large‐effect QTL were identified controlling HPPD‐inhibitor resistance in A. tuberculatus . This is the first QTL mapping study to characterize herbicide resistance in a weedy species. Abstract : Genetic mapping of 4‐hydroxyphenylpyruvate dioxygenase inhibitor resistance in a population of Amaranthus tuberculatus from Nebraska in the pseudo‐F2 generation identified two quantitative loci underlying the trait. © 2021 Society of Chemical Industry.