The tumour microenvironment influences survival and time to transformation in follicular lymphoma in the rituximab era. (24th June 2016)

• Record Type: Journal Article
• Authors: Blaker, Yngvild Nuvin; Spetalen, Signe; Brodtkorb, Marianne; Lingjærde, Ole Christian; Beiske, Klaus; Østenstad, Bjørn; Sander, Birgitta; Wahlin, Björn Engelbrekt; Melen, Christopher Michael; Myklebust, June Helen; Holte, Harald; Delabie, Jan; Smeland, Erlend Bremertun
• Is Part Of: British journal of haematology. Volume 175:Number 1(2016)
• Page Start: 102
• Page End: 114
• Publication Date: 2016-06-24
• Subjects: follicular lymphoma -- tumour microenvironment -- follicular dendritic cell -- immunohistochemistry -- prognostic factors; Hematology -- Periodicals; Blood -- Diseases -- Periodicals; 616.15
• DOI: 10.1111/bjh.14201
• Languages: English
• ISSNs: 0007-1048

Abstract

Summary: The tumour microenvironment influences outcome in patients with follicular lymphoma (FL), but its impact on transformation is less studied. We investigated the prognostic significance of the tumour microenvironment on transformation and survival in FL patients treated in the rituximab era. We examined diagnostic and transformed biopsies from 52 FL patients using antibodies against CD3, CD4, CD8, CD21 (CR2), CD57 (B3GAT1), CD68, FOXP3, TIA1, PD‐1 (PDCD1), PD‐L1 (CD274) and PAX5. Results were compared with a second cohort of 40 FL patients without signs of transformation during a minimum of five years observation time. Cell numbers and localization were semi‐quantitatively assessed. Better developed CD21+ follicular dendritic cell (FDC) meshworks at diagnosis was a negative prognostic factor for overall survival (OS), progression‐free survival (PFS) and time to transformation (TTT) in patients with subsequently transformed FL. Remnants of FDC meshworks at transformation were associated with shorter OS and PFS from transformation. High degrees of intrafollicular CD68+ and PD‐L1+ macrophage infiltration, high total area scores and an extrafollicular/diffuse pattern of FOXP3+ T cells and high intrafollicular scores of CD4+ T cells at diagnosis were associated with shorter TTT. Scores of several T‐cell subset markers from the combined patient cohorts were predictive for transformation, especially CD4 and CD57.