Pseudolaric acid B ameliorates synovial inflammation and vessel formation by stabilizing PPARγ to inhibit NF‐κB signalling pathway. Issue 14 (11th June 2021)

• Record Type: Journal Article
• Authors: Lu, Jiansen; Guan, Hong; Wu, Dan; Hu, Zhiqiang; Zhang, Hongbo; Jiang, Huaji; Yu, Jingyao; Zeng, Ke; Li, Hongyu; Zhang, Haiyan; Pan, Chenglong; Cai, Daozhang; Yu, Xiao
• Is Part Of: Journal of cellular and molecular medicine. Volume 25:Issue 14(2021)
• Page Start: 6664
• Page End: 6678
• Publication Date: 2021-06-11
• Subjects: NF‐κB signalling -- Osteoarthritis -- PPARγ -- pseudolaric acid B; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.16670
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: Synovial macrophage polarization and inflammation are essential for osteoarthritis (OA) development, yet the molecular mechanisms and regulation responsible for the pathogenesis are still poorly understood. Here, we report that pseudolaric acid B (PAB) attenuated articular cartilage degeneration and synovitis during OA. PAB, a diterpene acid, specifically inhibited NF‐κB signalling and reduced the production of pro‐inflammatory cytokines, which further decreased M1 polarization and vessel formation. We further provide in vivo and in vitro evidences that PAB suppressed NF‐κB signalling by stabilizing PPARγ. Using PPARγ antagonist could abolish anti‐inflammatory effect of PAB and rescue the activation of NF‐κB signalling during OA. Our findings identify a previously unrecognized role of PAB in the regulation of OA and provide mechanisms by which PAB regulates NF‐κB signalling through PPARγ, which further suggest targeting synovial inflammation or inhibiting vessel formation at early stage could be an effective preventive strategy for OA.