Preparation of Artificial Hexaplex Composed of Non‐Natural Nucleic Acid. Issue 4 (13th April 2021)

• Record Type: Journal Article
• Authors: Makino, Koki; Hattori, Yuhei; Kashida, Hiromu; Asanuma, Hiroyuki
• Is Part Of: Current protocols. Volume 1:Issue 4(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-04-13
• Subjects: aminopyrimidine -- cyanuric acid -- D‐threoninol nucleic acid -- hexaplex -- triaminopyrimidine; Life sciences -- Laboratory manuals -- Periodicals; Biology -- Laboratory manuals -- Periodicals; Life sciences -- Technique -- Periodicals; Biology -- Technique -- Periodicals; 570.028
• DOI: 10.1002/cpz1.106
• Languages: English
• ISSNs: 2691-1299

Abstract

Abstract: This article describes synthetic procedures for acyclic D ‐threoninol nucleic acid tethering of bifacial nucleobases. Because these nucleobases have complementary hydrogen bonding sites on both sides, their oligomers can form a hexaplex. These hexaplexes are suitable for use as metal or pH sensors and as supramolecular motifs. © 2021 Wiley Periodicals LLC. Basic Protocol 1 : Synthesis of D ‐threoninol backbone Basic Protocol 2 : Synthesis of D ‐ a TNA tethering cyanuric acid Basic Protocol 3 : Synthesis of D ‐ a TNA tethering a 2‐aminopyrimidine moiety Basic Protocol 4 : Synthesis of D ‐ a TNA tethering a 2, 4, 6‐triaminopyrimidine moiety Basic Protocol 5 : Synthesis of D ‐ a TNA oligomer tethering cyanuric acid, 2‐aminopyrimidine, or 2, 4, 6‐triaminopyrimidine